Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds

Abstract

The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the
evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2
content using a drag system and exhaustion to the gas released by the seeds. Samples of 50 seeds of six lots of cultivar
CD 123 were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of
incubation were quantified. Additionally, the percentage of normal seedlings emerged in the field were evaluated. The
simple correlation coefficients were evaluated among tests. After determining the reading conditions, the precision and
accuracy of the proposed method were evaluated, using 15 lots of seeds. For the evaluation of the respiratory activity
in Triticum aestivum seeds, a sample of 25 seeds, incubated at 25 °C for a minimum of 12 h is recommended since it allows
to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate
method for measuring CO2 as it externalizes precision between successive measurements and agreement with the
reference method.