Use of Maldi-Tof MS biosensor in Brazilian kefir grains microbial assessment


  • Roberta Oliveira Viana
  • Karina Teixeira Magalhães-Guedes Escola de Nutrição, Universidade Federal da Bahia - UFBA, Campus Canela Cep: 40.110-150 - Salvador - BA - Brasil
  • Disney Ribeiro Dias
  • Rosane Freitas Schwan


microorganisms, bacteria’s, yeast, biosensor


The aim of this study was to identify the microorganisms associated with Brazilian kefir grains using the a new methodology to microorganisms rapid diagnosis, Maldi-Tof MS biosensor. A total of 357 microorganisms were isolated, and 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria’s). The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii. Rapid diagnosis of microorganisms is decisive to guarante adequate identification in biológical samples. Maldi-Tof biosensor is available to enable faster diagnosis for microbial identification. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using this biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.


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