Microanálise em trabalhos de rotina

II - Avaliação de proteínas

Autores

  • Walter Brune UFV
  • José Mário Braga UFV
  • Cid Martins Batista UFV
  • Dirceu Jorge da Silva UFV

Palavras-chave:

processo kjeldahl, erro experimental, análise química, transporte de amoníaco, microdifusão de amônio

Resumo

Three forms of the Kjeldahl process were compared with one another to determine which would be most suitable for routine work: 'Macroanalysis', in which ammonium was distilled; 'Semi- microanalysis', in which ammonium was transported by vapor; and 'Microanalysis', in which ammonium was transported by dif- fussion (Conway). 

Referências

ARCHIBALD, R.M. Nitrogen by the Kjeldahl method. In: REINER, M. ed. Standard methods of clinical chemistry New York, Academic Press, 1958. V. 2. P. 91-9

BEACH, E. Nonprotein nitrogen. In: REINER, M. ed. Standard methods of clinical chemistry. New York,Academic Press, 1958. V. 2. P. 100-6.

CHRISTIAN, Gary D. Analytical chemistry. Waltham, Mass., Xerox College Publishing, 1971. 508 p.

CONWAY, Edward J. Microdiffusion analyses and volumetric error. New York, Chemical Publishing, 1963. 467 p.

CONWAY, Edward J. § O'MALLEY, E. Microdiffusion methods. Ammonia and urea using buffered absorbents.The Biochemi- cal Journal, London, 36(7):655-61. 1942.

DOYLE, W.L. § OMOTO, J.H. Ultramicrodetermination of ni- trogen. Analytical Chemistry, Washington, D.C. 22(4): 603-4. 1950.

IMPROVED Kjeldahl method for nitrate-containing samples. In: HORWITZ, William. ed. Official methods of analyses of the Association of Official Analytical Chemestry. 11. ed. Washington, AOAC, 1970. cap. 2, p. 17(n. 2.052.).

KONIG, I.K. Untersuchung von Nahrungs, Genussmitteln und Gebrauchsgegenstdnden. 4. ed. Berlin, Springer Verl, 1910. 772 p.

KUCK, J.A.; KINGSLEY, A.; KINSEY, D.; SHEEHAN, F.;SWIGERT, G. F. Kjeldahl ultramicrodeterminantion of nitrogen. Analytical Chemistry, Washington,D.C. 22(4):604-11.1950

PREGL, Fritz § ROTH, Hubert. Die quantitative organische Mikroanalyses. Berlin, Verlag Julius Springer, 1935. 328 p.

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Publicado

2025-03-19

Como Citar

Brune, W., Braga, J. M., Martins Batista, C., & da Silva, D. J. (2025). Microanálise em trabalhos de rotina: II - Avaliação de proteínas. Revista Ceres, 22(121), 178–186. Recuperado de https://ojs.ceres.ufv.br/ceres/article/view/6818

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