Efeito do Glifosato no cultivo in vitro de Cattleya nobilior Rchb. F
Palavras-chave:
hormone, micropropagation, OrchidaceaeResumo
Among the techniques used by biotechnology for multiplication of orchid seedlings, the in vitro cultivation stands out due to the high production potential of new seedlings. The presence of some chemical substances used in the culture medium, depending on the concentration, may or may not favor the growth and development of the plant. Therefore, the objective of this work was to evaluate the effect of glyphosate on the in vitro cultivation of the orchid Cattleya nobilior Rchb. F. The experimental design was completely randomized, with six treatments and ten replications. The treatments consisted of six concentrations of glyphosate (0.0, 8.5, 17.0, 25.5, 34.0, and 42.5 mg L-1 a.i.), added to the modified MS culture medium with half the concentrations of macronutrients. Seedlings subjected to different glyphosate concentrations were obtained from seeds germinated in vitro. The length and dry biomass measurements of the shoot and root, number of leaves, leaf length, leaf width, number of shoots, chlorophyll a, b, and carotenoid contents were performed 200 days after transplanting of the seedlings. Among the phytometric components analyzed, only the length and dry biomass of root and the carotenoid content decreased with increasing doses of the glyphosate herbicide. Low concentrations of glyphosate added to the culture medium result in hormese effect, with increased growth of Cattleya nobilior Rchb. F. shoot.
Referências
Baur JR (1979) Eftect of glyphosate on auxin transport in corn and cotton tissues. Plant Physiology, 63:882-886.
Benzing DH, Ott DW & Friedman WE (1982) Roots of Sobralia macrantha (Orchidaceae): structure and function of the velamenexodermis complex. American Journal of Botany, 69:508-614.
Calabrese EJ & Baldwin LA (2001) Hormesis: a generalizable and unifying hypothesis. Critical Reviews in Toxicology, 31:353-424.
Calabrese EJ, Baldwin LA & Holland CD (1999) Hormesis: A highly generalizable and reproducible phenomenon with important implications for risk assessment. Risk Analysis, 19:261-281.
Carbonari C, Meschede DK & Velini ED (2007) Efeitos da aplicação de glyphosate no crescimento inicial de mudas de eucalipto submetidas a dois níveis de adubação fosfatada. In: Simpósio Internacional sobre Glyphosate, Botucatu. Anais, FEPAF. p.68-70.
Carvalho LB & Alves PLC (2011) Efeitos do glyphosate na fotossíntese do cafeeiro. In: Simpósio Internacional sobre Glyphosate, Botucatu. Anais, FEPAF. p.86-89.
Cedergreen N (2008) Is the growth stimulation by low doses of glyphosate sustained over time? Environmental Pollution, 156:1099-1104.
Cedergreen N & Olesen CF (2010) Can glyphosate stimulate photosynthesis? Pesticide Biochemistry and Physiology, 96:140-148.
Cronquist A (1981) An integrated system of classification of flowering plants. New York, Columbia University Press. 1262p.
Duke SO, Cedergreen N, Velini ED & Belz RG (2006) Hormesis: Is it an important factor in herbicide use and allelopathy? Outlooks on Pest Management, 17:29-33.
Faria RT, Dalio RJD, Unemoto LK & Silva GL (2006) Propagação in vitro de Oncidium baueri Lindl. (Orchidaceae) sem uso de ágar. Acta Scientia Agronomica, 28:71-74.
Galli AJB & Montezuma MC (2005) Alguns aspectos da utilização de glyphosate na agricultura. São Paulo, ACADCOM Gráfica e Editora. 66p.
Godoy MC (2007) Efeitos do glyphosate sobre o crescimento e absorção de fósforo pela soja. Dissertação de Mestrado. Universidade Estadual Paulista, São Paulo. 53p.
Halter S (2009) História do herbicida agrícola glyphosate. In: Velini ED (Ed.) Glyphosate. Botucatu, FEPAF. p.11-16.
Kruse ND, Trezzi MM & Vidal RA (2000) Herbicidas inibidores da EPSPs: revisão de Literatura. Revista Brasileira de Herbicidas, 1:139-146.
Mayer JLS, Ribas LLF, Bona C & Quoirin M (2008) Anatomia comparada das folhas e raízes de Cymbidium Hort. (Orchidaceae) cultivadas ex vitro e in vitro. Acta Botânica Brasileira, 22:323-332.
Meschede DK, Velini ED, Carbonari CA & Silva JRM (2011) Alteração fisiológica da cana-de-açúcar pela aplicação de glyphosate e sulfumeturon-methyl. Planta Daninha, 1:413-419.
Meschede DK, Carbonari CA & Velini ED (2007) Efeito de subdoses de glyphosate sobre o crescimento e desenvolvimento de Commelina benghalensis. In: Simpósio Internacional Sobre Glyphosate, Botucatu. Anais, FEPAF. p.65-67.
Mousdale DM & Coggins JR (1991) Amino acid synthesis. In: Kirkwood RC (Ed.) Target sites for herbicide action. New York, Premium Press. 327p.
Murashige T & Skoog FA (1962) Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15:473-497.
Oliveira LM, Paiva R, Santana JRF, Nogueira RC, Soares FP & Silva LC (2007) Efeito de citocininas na senescência e abscisão foliar durante o cultivo in vitro de Annona glabra L. Revista Brasileira de Fruticultura, 29:25-30.
Pereira CS, Bevilacqua UC, Souza TV, Matte WD & Chapla MV (2016) Phytotoxicity in transgenic soybean treated with glyphosate doses. Scientific Electronic Archives, 3:52-61.
Pierik RLM (1990) Cultivo in vitro de las plantas superiores. 3ª ed. Madrid, Ediciones Mundi-Prensa. 120p.
Pincelli-Souza RP (2014) Hormesis de glyphosate em cana-deaçúcar. Tese de Doutorado. Universidade Estadual Paulista, São Paulo. 75p.
R Development Core Team (2012) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Disponível em: <http://www.Rproject.org/>. Acessado em: 20 de abril de 2017.
Ross J & O’Neil D (2001) New interactions between classical plant hormones. Trends in Plant Science, 6:2-4.
Schmidt RR (1997) Classification of herbicides according to mode of action. In: Brigton Crop Protection Conference – Weeds, Brighton. Proceedings, HRAC. p.1133-1140.
Silva MDA, Aragão NC, Barbosa MDA, Jeronimo EM & Carlin SD (2009) Efeito hormótico de gliphosate no desenvolvimento inicial de cana-de-açúcar. Bragantia, 1:973-978.
Sorace M, Faria RT, Batista Fonseca IC, Yamamoto LY & Fonseca Sorace MA (2009) Alternative substrata for xaxim on culture of Cattleya intermedia X Hadrolaelia purpurata (Orchidaceae). Semina: Ciências Agrárias, 30:771-778.
Stancato GC & Faria RT (1996) In vitro growth and mineral nutrition of the lithophytic orchid Laelia cinnabarina Batem. (Orchidaceae) I: effects of macro and microelements. Lindleyana, 11:41--43.
Taiz L & Zeiger E (2013) Fisiologia vegetal. 5ª ed. Porto Alegre, Artmed. 918p.
Takane RJ, Yanagisawa SS & Pivetta KFL (2010) Cultivo moderno de orquídeas: Cattleya e seus híbridos. Fortaleza, UFC. 179p.
Tomaz HVQ (2009) Fontes, doses e formas de aplicação de fósforo na cana-de-açúcar. Dissertação de Mestrado. Escola Superior de Agricultura “Luiz de Queiroz”, São Paulo. 93p.
Velini ED, Carbonari ED, Meschede DK & Trindade MLB (2012) Modo de ação do glyphosate. In: Velini ED, Carbonari ED, Meschede DK & Trindade MLB (Eds.) Glyphosate: uso sustentável. Botucatu, FEPAF. 213p.
Velini ED, Alves E, Godoy MC, Meschede DK, Souza RT & Duke SO (2008) Glyphosate applied at low doses can stimulate plant growth. Pest Management Science, 64:489-496.
Vitti GC, Oliveira DB & Quintino TA (2006) Micronutrientes na cultura da cana-de-açúcar. In: Segato SV, Pinto AS, Jendiroba E & Nóbrega JCM (Eds.) Atualização em produção de cana-deaçúcar. p.121-138.
Yamada T & Castro PBC (2007) Efeitos do glifosato nas plantas: implicações fisiológicas e agronômicas implicações fisiológicas e agronômicas. International Plant Nutrition Institute, 1:1-32.
Yoshida S (1969) Biosynthesis and conversion of aromatic amino acids in plants. Annual Review Plant Physiology, 20:41-62.
Wagner R, Kogan M & Parada AM (2003) Phytotoxic activity of root absorbed glyphosate in corn seedlings (Zea mays L.). Weed Biology Management, 3:228-232.
Watanabe D (2002) Orchidas: manual de cultivo. São Paulo, Associação Orquidófila de São Paulo. 296p.
Whitham FH, Blaydes DF & Devlin RM (1971) Experiment in plant physiology. New York, Van Nostrand Company. 236p.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
