Antimicrobial and antibiofilm activity of Cymbopogon flexuosus essential oil microemulsions
Palavras-chave:
biofilm, plants antibacterial, disinfectant, secondary plant metabolism, bacterial pathogensResumo
The objective of the study was to determine the antimicrobial activity antibiofilm and to identify the main components of the essential oil (EO) obtained from the leaves of Cymbopogon flexuosus. The antibacterial and antibiofilm activity was determined against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Salmonella Typhimurium ATCC 14028 and Listeria monocytogenes ATCC 19117. The effect of EO on biofilm was evaluated by quantifying viable cell number (CFU) and biomass by crystal violet (CV) analysis. The composition of the essential oils was determined by GC / FID and GC / MS. The results showed action against L. monocytogenes, S. aureus and S. Typhimurium with MIC and MBC values of 3.9 µL mL-1, thus showing satisfactory antimicrobial activity, given this was the lowest concentration tested. For the antibiofilm activity, a significant reduction (P < 0.05) was observed for S. typhimurium and S. aureus. Biofilm biomass significantly reduced only for S. aureus and P. aeruginosa. EO presented the geranial and neral isomers as major components.
Referências
Ayala-Zavala JF, González-Aguilar GA & Del-Toro-Sánchez L (2009) Enhancing safety and aroma appealing of fresh-cut fruits and vegetables using the antimicrobial and aromatic power of essential oils. Journal of Food Science, 74:84-91.
Bakkali F, Averbeck S, Averbeck D & Idaomar M (2008) Biological effects of essential oils: a review. Food and Chemical Toxicology, 46:446-75.
Bazargani MM & Rohloff J (2016) Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms. Food Control, 61:156-64.
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods – a review. International Journal of Food Microbiology, 94:223-253.
Celiktas OY, Kocabas EEH, Bedir E, Sukan FV & Baser KHC (2007) Antimicrobial activity of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry, 100:553-559.
Chavant P, Gaillard-Mertinie B, Talon R, Hébraud M & Bernardi T (2007) A new device for rapid evaluation of biofilm formation potential by bacteria. Journal of Microbiological Methods, 68:605-612.
Costerton JW (2005) Biofilm theory can guide the treatment of device-related orthopedic infections. Clinical Orthopaedics and Related Research, 437:07-11.
Dalla Costa KA, Ferenz M, Silveira SM & Millezi AF (2016) Formação de biofilmes bacterianos em diferentes superfícies de indústrias de alimentos. Revista Instituto Laticínios Cândido Tostes, 71:75-82.
Donlan RM (2002) Biofilms: Microbial Life on Surfaces. Emerging Infectious Diseases, 8:881-890.
Donlan RM & Costerton JW (2002) Biofilms: survival mechanisms of clinically relevant microorganisms. Clinical Microbiology Revision, 15:167-93.
El-Sayed AM (2018) The Pherobase: Database of Pheromones and Semiochemicals. Available at: <http://www.pherobase.com>. Accessed on: August 24th, 2018.
Ganjewala D & Gupta AK (2013) Lemongrass (Cymbopogon flexuosus Steud.) Essential Oil: Overview and Biological Activities. Recent Progresses in Medicinal Plants, 37:234-74.
Ganjewala D, Kumari A & Khan KH (2008) Ontogenic and developmental changes in essential oil content and compositions in Cymbopogon flexuosus cultivars. In: Prasad BN & Lazer Mathew (Eds.) Recent Advance in Biotechnology. New Delhi, Excel India Publishers. p.82-92.
Hall-Stoodley L, Costerton JW & Stoodley P (2004) Bacterial biofilms: from the natural environment to infectious diseases. Natural Research Microbiology, 2:95-108.
Kumar R, Srivastava M & Dubey NK (2007) Evaluation of Cymbopogon martini oil extract for control of postharvest insect deterioration in cereals and legumes. Journal of Food Protection, 270:172-178.
Kumar S, Subudhi E, Nayak S & Sahoo S (2009) Differential citral content of 15 lemongrass genotypes and their antimicrobial property. Internet Journal of Microbiology, 6:01-08.
Junker LM & Clardy J (2007) High-throughput screens for smallmolecule inhibitors of Pseudomonas aeruginosa biofilm development. Antimicrobial Agents and Chemotherapy, 51:3582-3590.
Millezi AF, Pereira MO, Batista NN, Camargos N, Auad I, Cardoso MDG & Piccoli RH (2012) Susceptibility of mono and dualspecies biofilms of Staphylococcus aureus and Escherichia coli to essential oils. Journal of Food Safety, 32:351-359.
Millezi AF, Piccoli RH, Oliveira JM & Pereira MO (2016) Antibiofilm and Antibacterial Effect of Essential Oils and Their Major Compounds. Journal of Essential Oil Bearing Plants, 19:624-31.
Moreira MR, Ponce AG, Del Valle CE & Roura SI (2005) Inhibitory parameters of essential oils to reduce a foodborne pathogen. ý Food Science and Technology, 38:565-570.
Nascimento GGF, Locatelli J & Freitas PC (2000) Antibacterial activity of plant extracts and phytochemicals on antibioticresistant bacteria. Brazilian Journal of Microbiology, 31:247-56.
NCCLS/CLSI (2003) M07-A10- Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. 6th Edition. Wayne, NCCLS/CLSI. 87p.
Nguyen UT, Wenderska IB, Chong MA, Koteva K, Wright GD & Burrows LL (2012) Small-molecule modulators of Listeria monocytogenes biofilm development. Applied and Environmental Microbiology, 78:1454-1465.
Oliveira MMM, Brugnera DF, Nascimento JA & Piccoli RH (2012) Control of planktonic and sessile bacterial cells by essential oils. Food and Bioproducts Processing, 90:809-18.
Prigent-Combaret C & Lejeune P (1999) Monitoring gene expression. In biofilms. Methods in Enzymology, 310:56-79.
Rota CM, Herrera A, Martínez RM, Sotomayor JA & Jordán MJ (2008) Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control, 19:681-687.
Santoro GF, Cardoso MG, Guimaraes LG, Freire JM & Soares MJ (2007) Antiproliferative effect of the essential oil of Cymbopogon citratus (DC) Stapf (lemongrass) on intracellular amastigotes, bloodstream trypomastigotes and culture epimastigotes of Trypanosoma cruzi (Protozoa: Kinetoplastida). Parasitology, 134:1649-1656.
Sharma PR, Mondhe DM, Muthiah S, Pal HC, Shahi AK & Saxena AK (2009) Anticancer activity of essential oil from Cymbopogon flexuosus. Chemical-Biological Interactions, 179:160-168.
Simões M, Dias C, Borge A & Saavedra MJ (2018) Biofilm formation and multidrug-resistant Aeromonas spp. from wild animals. Journal of Global Antimicrobial Resistance, 12:227-234.
Singh N, Luthra R & Sangwan RS (1989) Effect of leaf position and age on the essential quantity and quality in lemongrass (Cymbopogon flexusosus Stapf.). Planta Medica, 55:254-256.
Stepanovic S, Vucovic D, Dakic I & Savic B (2000) A modified microtiter-pate test for quantification of staphylococcal biofilm formation. Journal of Microbiological Methods 40:175-179.
Xiaofeng Z, Guo Y, Guo L, Jiang H & Ji Q (2018) In Vitro Evaluation of Antioxidant and Antimicrobial Activities of Melaleuca alternifolia Essential Oil. BioMed Research International, p.01-08.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
