Phytotoxic potential of aqueous leaf extract of Tocoyena formosa and Rudgea viburnoides
Palavras-chave:
Lactuca sativa L., bioassays, aqueous extract, germinationResumo
Plants have organic components produced as secondary metabolites able to positively or negatively interfere on normal development of other species. The objective of this research was to determine the phytotoxic effects of aqueous leaf extract from two species Rubiaceae family: Tocoyena formosa and Rudgea viburnoides on the germination and initial development of lettuce (Lactuca sativa L). The extract was obtained from mixing 50 g of fresh leaf and 500 mL of distilled water. The experiment was arranged in completely randomized design for each specie, subjected to five extract concentrations: 0, 25, 50, 75 and 100%; and 4 repetitions of 50 seed each. The bioassays were performed in laboratory at controlled temperature and luminosity during seven days. Results showed that the aqueous extract of both species do not affected the germination and the percent of abnormal lettuce seedlings. R. viburnoides showed no pronounced difference in relation to shoot length and dry biomass; however, the root length was reduced by 85%. The extract of T. formosa at higher concentrations, drastically reduce the dry biomass and length of shoots and roots. Therefore, both species have phytotoxic effect on seedling growth of lettuce being the length root the most affected variable.
Referências
Almeida JMAD (2011) Potencial das folhas de Rudgea viburnoides (cham.) benth (rubiaceae) no tratamento da obesidade e suas alterações metabólicas induzidas por dieta em Camundongos Balb/c. Dissertação de Mestrado. Universidade Federal de Minas Gerais, Belo Horizonte. 119p.
Alves RMS, Stehmann JR, Isaías RMS & Brandão MGL (2004) Caracterização botânica e química de Rudgea viburnoides (Cham.) Benth., (Rubiaceae). Revista Brasileira de Farmacognosia, 14:49-56.
Ayers RS & Westcot DW (1985) Water quality for agriculture. Rome, Food and Agriculture Organization of the United Nations. 97p.
Barbosa MR, Zappi D, Taylor C, Cabral E, Jardim JG, Pereira MS, Calió MF, Pessoa MCR, Salas R, Souza EB, Di Maio FR, Macias L, Anunciação EA, da Germano Filho P, Oliveira JA, Bruniera CP, Gomes M, De Toni K & Firens M (2015) Rubiaceae in Lista de Espécies da Flora do Brasil. Disponível em: <http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB210>. Accessed on: September 18th, 2018.
Batish DR, Singh HP, Setia N, Kohli RK, Kaur S & Yadav SS (2007) Alternative control of littleseed canary grass using eucalypt oil. Agronomy for Sustainable Development, 27:171-177.
Brasil (2009) Regras para análise de sementes. Brasilia, MAPA / ACS. 395p.
Cesário FRAS, Albuquerque TRD, Lacerda TMD, Oliveira MRCD, Silva BAFD, Rodrigues LB, Martins AOBPBM, Almeida JRSG, Vale ML, Coutinho HDM & Menezes IRAD (2018a) Chemical fingerprint, acute oral toxicity and anti-inflammatory activity of the hydroalcoholic extract of leaves from Tocoyena formosa (Cham. and Schlecht.) K. Schum. Saudi Journal of Biological Sciences, 26:01-08.
Cesário FRAS, de Albuquerque TR, de Lacerda GM, de Oliveira MRC, Rodrigues LB, Martins AOBPB, Boligon AA, Júnior LJQ, Araújo AADS, Vale ML, Coutinho HDM & Menezes IRAD (2018b) Phytochemical profile and mechanisms involved in the anti-nociception caused by the hydroethanolic extract obtained from Tocoyena formosa (Cham. & Schltdl.) K. Schum (Jenipapo-bravo) leaves in mice. Biomedicine & Pharmacotherapy, 97:321-329.
Chachalis D & Reddy KN (2000) Factors affecting Campsis radicans seed germination and seedling emergence. Weed Science, 48:212-216.
Chung IM, Ahn JK & Yun SJ (2001) Assessment of allelopathic potential of barnyard grass (Echinochloa crus-galli) on rice (Oryza sativa L.) cultivars. Crop protection, 20:921-928.
Delprete PG (2008) Revision of Tocoyena (Rubiaceae: Gardenieae) from the states of Goiás and Tocantins and a new species endemic to white-sand areas in the brazilian cerrado. Journal of the Botanical Research Institute of Texas, 2:983-993.
Ferreira AG & Aquila MEA (2000) Alelopatia: uma área emergente da ecofisiologia. Revista Brasileira de Fisiología Vegetal, 12:175-204.
Ferreira AG (2004) Interferência: competição e alelopatia. In: Ferreira AG & Borghetti F (Eds.) Germinação: do básico ao aplicado. Porto Alegre, Artmed. p. 251-262.
Ferreira DF (2010) Programa computacional Sisvar. Versão 5,6. Lavras, UFLA. CD-ROM.
Frescura VDS, Kuhn AW, Laughinghouse IV HD, Nicoloso FT, Lopes SJ & Tedesco SB (2013) Evaluation of the allelopathic, genotoxic, and antiproliferative effect of the medicinal species Psychotria brachypoda and Psychotria birotula (Rubiaceae) on the germination and cell division of Eruca sativa (Brassicaceae). Caryologia, 66:138-144.
Galdino PM, Alexandre LN, Pacheco LF, Junior RDSL, de Paula JR, Pedrino GR, Xavier CE & Ferreira PM (2017) Nephroprotective effect of Rudgea viburnoides (Cham.) Benth leaves on gentamicin-induced nephrotoxicity in rats. Journal of ethnopharmacology, 201:100-107.
Gatti AB, Takao LK, Pereira VC, Ferreira AG, Lima MIS & Gualtieri SCJ (2014) Seasonality effect on the allelopathy of cerrado species. Brazilian Journal of Biology, 74:64-69.
Gatti AB, Perez SCJGA & Lima MIS (2004) Atividade alelopática de extratos aquosos de Aristolochia esperanzae O. Kuntze na germinação e no crescimento de Lactuca sativa L. e Raphanus sativus L. Acta Botânica Brasílica, 18:459-472.
Golisz A, Lata B, Gawronski SW & Fujii Y (2007) Specific and total activities of the allelochemicals identified in buckwheat. Weed Biology and Management, 7:164-171.
Gusman GS, Vieira LR & Vestena S (2012) Alelopatia de espécies vegetais com importância farmacêutica para espécies cultivadas. Biotemas, 25:37-48.
Iqbal Z, Hiradate S, Noda A, Isojima Si & Fujii Y (2003) Allelopathic activity of buckwheat: isolation and characterization of phenolics. Weed science, 51:657-662.
Magiero EC, Assmann JM, Marchese JA, Capelin D, Paladini MV & Trezzi MM (2009) Efeito alelopático de Artemisia annua L. na germinação e desenvolvimento inicial de plântulas de alface (Lactuca sativa L.) e leiteiro (Euphorbia heterophylla L.). Revista Brasileira de Plantas Medicinais, 11:317-324.
Maguire JD (1962) Speed of germination-aid in selection evaluation for seedling emergence and vigor. Crop Science, 2:176-177.
Malta PG, Arcanjo-Silva S, Ribeiro C, Campos NV & Azevedo AA (2016) Rudgea viburnoides (Rubiaceae) overcomes the low soil fertility of the Brazilian Cerrado and hyperaccumulates aluminum in cell walls and chloroplasts. Plant and soil, 408:369-384.
Matias R, Oliveira AKM, Pereira KCL, Rizzi ES & Rosa AC (2018) Potencial alelopático do extrato etanólico de Anacardium humile (cajuzinho-do-cerrado) na germinação e formação de plântulas de alface, tomate e fedegoso. Gaia Scientia, 12:144-160.
Oliveira AK, Pereira KC, Muller JA & Matias R (2014a) Análise fitoquímica e potencial alelopático das cascas de Pouteria ramiflora na germinação de alface. Horticultura Brasileira, 32:41-47.
Oliveira AKMD, Matias R, Lopes SS & Fontoura FM (2014b) Allelopathy and influence of leaves of Palicourea rigida (Rubiaceae) on seed germination and seedling formation in lettuce. Bioscience Journal, 30:938-947.
Oliveira J, Peixoto C, Poelking V & Almeida A (2015) Avaliação de extratos das espécies Helianthus annuus, Brachiaria brizanthae, Sorghum bicolor com potencial alelopático para uso como herbicida natural. Revista Brasileira de Plantas Medicinais, 17:379-384.
Pereira JC, de Albuquerque Paulino CL, da Silva Granja B, Santana AEG, Endres L & Souza RC (2018) Potencial alelopático e identificação dos metabólitos secundários em extratos de Canavalia ensiformis L. Revista Ceres, 65:243-252.
Pires NM & Oliveira VR (2011) Alelopatia. In: Oliveira Jr. RS, Constantin J & Inoue MH (Eds.) Biologia e Manejo de Plantas Daninhas. Curitiba, Omnipax. p.95-123.
Quispe FE, Ruíz RE, Isidrón MP, García MR & Santana RC (2010) Efecto alelopático de los extractos acuosos de Tectona grandis L. y Tagetes erecta L. sobre la germinación de cultivos de interés agrícola. Centro Agrícola, 37:61-66.
Santos LBD, Souza JK, Papassoni B, Borges DGL, Junior GAD, Souza JMED, Carollo CA & Borges FDA (2013) Efficacy of extracts from plants of the Brazilian Pantanal against Rhipicephalus (Boophilus) microplus. Revista Brasileira de Parasitologia Veterinária, 22:532-538.
Santos VHMD, Daneluzzi GS, Silva LP & Silva RMGD (2015) Evaluation of allelopathic potential of leaf extract of Kielmeyera coriacea on Lactuca sativa L. Bioscience Journal, 31:259-267.
Silva Júnior MC & dos Santos GC (2005) 100 árvores do cerrado: guia de campo. Rede de sementes do Cerrado, Brasília. 278p.
Silveira PF, Maia SSS & Coelho MFB (2012) Potencial alelopático do extrato aquoso de cascas de jurema preta no desenvolvimento inicial de alface. Revista Caatinga, 25:20-27.
Soltys D, Krasuska U, Bogatek R & Gniazdowska A (2013) Allelochemicals as bioherbicides — present and perspectives. IntechOpen, 2013:517-542.
Williamson GB & Richardson D (1988) Bioassays for allelopathy: measuring treatment responses with independent controls. Journal of chemical ecology, 14:181-187.
Young MCM, Araújo AR, da Silva CA, Lopes MN, Trevisan LMV & Bolzani VDS (1998) Triterpenes and Saponins from Rudgea viburnioides. Journal of Natural Products, 61:936-938.
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