Spontaneous vegetative propagules differentiation in Bowdichia virgilioides seedlings maintained at MS basal medium
Palavras-chave:
sucupira preta, putrescine, plant tissue cultureResumo
Bowdichia virgilioides Kunth is a medium size tree used in folk medicine and in furniture manufacturing which makes it a target of extractive processes. The aim of this work was to evaluate the endogenous content of polymines, amino acids, and sugars in seedlings of Bowdichia virgilioides that presented or not the development of spontaneously differentiated vegetative propagules in their roots. An average of thirty percent Bowdichia virgilioides in vitro growing plants cultivated in MS basal medium without growth regulators presented the differentiation of vegetative propagules. It was noted the propagules units presents embryo-like structures (globular - to leaf-shaped features) and these plants showed a compromised development when compared to the plants that do not presented these morphogenetic structures. Total sugars and amino acids content was significantly higher in the roots of plants with the vegetative propagules. With regard to polyamines content, there was no statistical difference, for spermine and spermidine. However, higher levels of putrescine were found in plants developing vegetative propagules. In this way, these results showed that this event are related to the amino acids, sugars and polyamines, mainly putrescine content.
Referências
Albuquerque KA, Guimarães RM, Almeida IF & Clemente ADCS (2007) Métodos para superação da dormência em sementes de sucupira-preta (Bowdichia virgilioides Kunth). Ciência e Agrotecnologia, 31:1716-1721.
Alcázar R & Tiburcio AF (2014) Plant polyamines in stress and devopment: an emerging area of research in plant sciences. Frontiers in Plant Science II, 5:319.
Aragão VPM, de Souza Ribeiro YR, Reis RS, Macedo AF, Floh EIS, Silveira V & Santa-Catarina C (2016) In vitro organogenesis of Cedrela fissilis Vell. (Meliaceae): the involvement of endogenous polyamines and carbohydrates on shoot development. Plant Cell, Tissue and Organ Culture, 124:611-620.
Arantes CS, Do Prado Júnior JÁ, Sousa JR, Do Vale VS & De Oliveira RMC (2015) Ação Facilitadora de Bowdichia virgilioides Kunth (Fabaceae) na Colonização de Espécies em uma Área de Cerrado Sentido Restrito. Caminhos de Geografia, 16:15-26.
Ashraf M, Akram NA, Al-Qurainy F & Foolad MR (2011) Drought tolerance: roles of organic osmolytes, growth regulators and mineral nutrients. Advances in Agronomy, 111:249-296.
Asthana P, Rai MK & Jaiswal U (2017) Somatic embryogenesis from sepal explants in Sapindus trifoliatus, a plant valuable in herbal soap industry. Industrial Crops and Products, 100:228-235.
Cheng WH, Wang FL, Cheng XQ, Zhu QH, Sun YQ, Zhu HG & Sun J (2015) Polyamine and it metobolite H2O2 play a key role in the conversion of embriogenic callus into somatic embryos in upland cotton. Frontiers in Plant Science, 6:1063.
Choffe KL, Murch SJ & Saxena PK (2000) Regeneration of Echinacea purpurea: induction of root organogenesis from hypocotyl and cotyledon explants. Plant Cell, Tissue and Organ Culture, 62:227-234.
Da Cruz ACF, Rocha DI, Iarema L, Ventrella MC, Costa MGC, Neto VBP & Otoni WC (2014) In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae). In Vitro Cellular & Developmental Biology-Plant, 50:76-83.
Da Silva CV, de Oliveira LS, Loriato VAP, da Silva LC, de Campos JMS, Viccini LF & Otoni WC (2011) Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passionfruit species, P. cincinnata Masters. Plant Cell, Tissue and Organ Culture (PCTOC), 107:407-416.
Fehér A (2015) Somatic embryogenesis-stress-induced remodeling of plant cell fate. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1849:385-402, 2015.
Feher A, Pasternak TP & Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell, Tissue and Organ Culture, 74:201-228.
Grzyb M, Kalandyk A, Waligórski P & Mikula A (2017) The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb. Plant Cell, Tissue and Organ Culture, 129:387-397.
Haensch KT (2004) Morpho-histological study of somatic embryolike structures in hypocotyl cultures of Pelargonium×hortorum Bailey. Plant Cell Reports, 22:376-381
Kaur A, Reddy MS & Kumar A (2018) Direct somatic embryogenesis of potato [Solanum tuberosum (L.)] cultivar ‘Kufri Chipsona 2’. Plant Cell, Tissue and Organ Culture, 134:457-466.
Mahdavi-darvari F, Noor NM, & Ismanizan I (2015) Epigenetic regulation and gene markers as signals of early somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 120:407-422.
Mahendran G & Bai VN (2016) Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze. Journal of Genetic Engineering and Biotechnology, 14:77-81.
Martin-Tanguy J (2001) Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regulation, 34:135-148.
Matam P & Parvatam G (2017). Putrescine and polyamine inhibitors in culture medium alter in vitro rooting response of Decalepis hamiltonii Wight & Arn. Plant Cell, Tissue and Organ Culture, 128:273-282.
Mose W, Indrianto A, Purwantoro A & Semiarti E (2017) The Influence of Thidiazuron on Direct Somatic Embryo Formation from Various Types of Explant in Phalaenopsis amabilis (L.) Blume Orchid. HAYATI Journal of Biosciences, 24:201-205.
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plantarum, 15:473-479.
Parimalan R, Giridhar P & Ravishankar GA (2011) Enhanced shoot organogenesis in Bixa orellana L. in the presence of putrescine and silver nitrate. Plant Cell, Tissue and Organ Culture, 105:285-290.
Reis RS, Vale EM, Heringer AS, Santa-Catarina C & Silveira V (2015) Putrescine induces somatic embryo development and proteomic changes in embryogenic callus of sugarcane. Journal of Proteomics, 130:170-179.
Roshanfekrrad M, Zarghami R, Hassani H, Zakizadeh H & Salari A (2017) Effect of AgNO3 and BAP on root as a novel explant in date palm (Phoenix dactylifera cv. Medjool) somatic embryogenesis. Pakistan Journal of Biological Sciences, 20:20-27.
Salvo AS, Hirsch CN, Buell CR, Kaeppler SM & Kaeppler HF (2014) Whole transcriptome profiling of maize during early somatic embryogenesis reveals altered expression of stress factors and embryogenesis-related genes. PLoS One, 9:e111407.
Shen HJ, Chen JT, Chung HH & Chang WC (2018) Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’. Botanical Studies, 59:4.
Silveira V, Floh EIS, Handro W & Guerra MP (2004) Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda. Plant Cell, Tissue and Organ Culture, 76:53-60.
Vila S, Gonzalez A, Rey H & Mroginski L (2005) Plant regeneration, origin, and development of shoot buds from root segments of Melia azedarach L. (Meliaceae) seedlings. In Vitro Cellular & Developmental Biology-Plant, 41:746.
Vondráková Z, Eliásová K, Vágner M, Martincová O & Cvikrova M (2015) Exogenous putrescine affects endogenous polyamine levels and the development of Picea abies somatic embryos. Plant Growth Regulation, 75:405-414.
Wei M, Wei SH & Yang CY (2010) Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots. Plant Cell, Tissue and Organ Culture, 102:145-151.
Yemm EW & Cocking EC (1955) The determination of aminoacids with ninhydrin. Analyst, 80:209-213.
Yemm EW & Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal, 57:508-514.
Downloads
Publicado
Como Citar
Edição
Seção
Licença

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.