Root morphology and kinetics of Zn absorption by roots of common bean influenced by Zn status of the root environment
Palavras-chave:
root Zn inflow;, absorption efficiency;, kinetic parameters;, Vmax;, Cmin;, Km.Resumo
Understanding the kinetics of Zn absorption by roots and its effect on morphology of this organ is relevant for improving crop management, but still poorly studied for common beans. Therefore, an experiment was conducted in a hydroponic system with five initial concentrations of Zn (CZnI): 0.0; 1.0; 4.0; 16.0 and 48.0 μmol L-1. The experiment was installed with plants at V3 stage of development and aliquots of the solution collected over 24 h. The maximum absorption rate (Vmax), Michaelis-Menten constant (Km) and the absorption power (α) increased as a function of CZnI. The minimum concentration of Zn estimated for its absorption (Cmin) was at 0.0028 mg L-1. The influx of Zn (Imax) was higher in higher CZnI, 16,0 μmol L-1. Root length, root volume, root Zn content and Zn absorption efficiency increased with the increase of CZnI. Therefore, the increase of CZnI positively influenced kinetic parameters of root Zn absorption and common bean root morphology, characteristics that favor Zn absorption by roots and improves overall plant nutrition, favoring agronomical biofortification practices for Zn and other nutrients.
Referências
Balk J, Connorton JM, Wan Y, Lovegrove A, Moore KL, Uauy C & Sharp PA (2019) Improving wheat as a source of iron and zinc for global nutrition. Nutrition Bulletin, 44:53-59.
Barber SA (1995) Soil Nutrient Bioavailability: A Mechanistic Approach. 2o ed. New York, John Wiley & Sons. 46p.
Batista RO, Eduardo A, Neto F, Fernanda S, Deccetti C & Viana CS (2016) Root morphology and nutrient uptake kinetics by australian cedar clones. Revista Caatinga, 29:153-162.
Blair WM (2013) Mineral Biofortification Strategies for Food Staples: The Example of Common Bean. Journal of Agricultural and Food Chemistry, 61:8287-8294.
Broughton WJ, Hernacutendez G, Blair M, Beebe S, Gepts P & Vanderleyden J (2003) Beans (Phaseolus spp.) endash; model food legumes. Plant and Soil, 252:55-128.
Cambraia TLL, Fontes RLF, Vergütz L, Vieira RF, Neves JCL, Netto PSC & Dias RF N (2019) Agronomic biofortification of common bean grain with zinc. Pesquisa Agropecuária Brasileira, 54:e01003.
Cakmak I (2008) Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant soil, 302:01-17.
Cakmak I & Kutman UB (2018) Agronomic biofortification of cereals with zinc: a review. European journal of soil science, 69:172-180.
Cambraia TL, Fontes RLF, Vergütz L, Vieira RF, Neves JCL, Corrêa Netto OS & Dias RFN (2019) Agronomic biofortification of common bean grain with zinc. Pesquisa Agropecuária Brasileira, 54, e01003.
Clark A (1997) Being there: Putting brain, body, and world together again. Cambridge, MIT Press. 292p.
Figueiredo MA, Boldrin PF, Hart JJ, de Andrade MJB, Guilherme LRG, Glahn RP & Li L (2017) Zinc and selenium accumulation and their effect on iron bioavailability in common bean seeds. Plant Physiology and Biochemistry, 111:193-202.
Epstein E & Hagen CE (1952) A kinetic study of the absorption of alkali cations by barley roots. Plant physiology, 27:457-474.
Fageria NK & Baligar CV (1997) Response of common bean, upland rice, corn, wheat, and soybean to soil fertility of an Oxisol. Journal of Plant Nutrition, 20:1279-1289.
Glass ADM, Britto DT, Kaiser BN, Kinghorn JR, Kronzucker J, Kumar A, Okamoto M, Rawat S, Siddiqi MY, Unkles E & Vidmar JJ (2002) The regulation of nitrate and ammonium transport systems in plants. Journal of Experimental Botany, 53:855-864.
Gupta N, Ram H & Kumar B (2016) Mechanism of Zinc absorption in plants: uptake, transport, translocation and accumulation.
Reviews in Environmental Science and Bio/Technology,15:89-109.
Hacisalihoglu G, Hart JJ & Kochian L V (2001) High and Low Affinity Zinc Transport Systems and Their Possible Role in Zinc Efficiency in Bread Wheat. Plant Physiology, 125:456- 463.
Hafeez B, Khanif YM & Saleem M (2013) Role of Zinc in Plant Nutrition- A Review. American Journal of Experimental Agriculture, 3:374-391.
Joseph C, Rathore VS, Bajaj YPS & Wittwer SH (1971) Mechanism of Zinc Absorption by Intact Bean Plants. Annals of Botany, 35:683-686.
Lee RB & Kathryn AR (1985) Effects of Nitrogen Deficiency on the Absorption of Nitrate and Ammonium by Barley Plants. Oxford Journals, 57:471-486.
Longnecker NE & Robson AD (1993) Distribution and Transport of Zinc in Plants. In: Robson AD (Ed.) Zinc in Soils and Plants. Developments in Plant and Soil Sciences. Dordrecht, Springer. p.79-91.
Malavolta E, Vitti GC & Oliveira AS (1997) Avaliação do estado nutricional das plantas: princípios e aplicações. Piracicaba, Associação Brasileira para Pesquisa da Potassa e do Fosfato. 319p.
Meng F, Liu D, Yang X, Shohag MJI, Yang J, Li T, Lu L & Feng Y (2014) Zinc uptake kinetics in the low and high-affinity systems of two contrasting rice genotypes. Journal of Plant Nutrition and Soil Science, 177:412-420.
Nie Z, Zhao P, Wang J, Li J & Liu H (2017) Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply. Frontiers in Plant Science, 8:01- 10.
Nye P & Tinker P (2000) The uptake properties of the root system. In: Press NYOU. Solute movement in the rhizosphere, 448p.
Patel PJ, Trivedi GR, Shah RK & Saraf M (2018) Selenorhi- zobacteria: As biofortification tool in sustainable agriculture. Biocatalysis and Agricultural Biotechnology, 14:198-203.
Pedas P, Hebbern CA, Schjoerring JK, Holm PE & Husted S (2005) Differential capacity for high-affinity manganese uptake contributes to differences between barley genotypes in tolerance to low manganese availability. Plant physiology,139:1411-1420.
Pedraza DF (2017) Estudos realizados no Brasil sobre a deficiência e a suplementação de zinco: ênfase em crianças. Revista Brasileira de Saúde Materna e Infantil, 17:233-249.
Pinto GM & Nazareth R (2016) Green synthesis and characterization of zinc oxide nanoparticles. Journal of Chemical and Pharmaceutical Research, 6:427-432.
Ram H, Rashid A, Zhang W, Duarte AP, Phattarakul N, Simunji S, Kalayci M, Freitas R, Rerkasem B, Bal RS, Mahmood K, Savasli E, Lungu O, Wang ZH, Barros VLNP, Malik SS, Arisoy RZ, Guo JX, Sohu VS, Zou CQ & Cakmak I (2016) Biofortification of wheat, rice and common bean by applying foliar zinc fertilizer along with pesticides in seven countries. Plant and Soil, 403:389- 401.
Rathore VS, Wittwer SH, Jyung WH, Bajaj YPS & Adams MW (1970) Mechanism of Zinc Uptake in Bean (Phaseolus vulgaris) Tissues. Physiologia Plantarum, 23:908-919.
Ribeiro Júnior JI(2001) Análises estatísticas no SAEG. Viçosa, UFV. 300p.
Rosolem CA, Sacramento LVS & Oliveira DMT (2005) Kinetics of zinc uptake and anatomy of roots and leaves of coffee trees as affected by zinc nutrition. Journal of Plant Nutrition, 28:2101-2112.
Riseman A & Craig R (2000). Physiological and morphological traits associated with zinc efficiency in Exacum. Plant Soil, 219:41-47.
Ruiz HA (1985) Estimativa dos parâmetros cinéticos Km e Vmax por uma aproximação gráfico-matemática. Revista Ceres, 32:79-84.
Ruiz HA & Fernandes Filho EI (1992) Cinética: Software para estimar as constantes Vmáx, Km da equação de Michaelis-Menten. In: Reunião brasileira de fertilidade do solo e nutrição de plantas. Anais, Piracicaba, Sociedade Brasileira de Ciência do Solo. p.124-125.
SAEG (2005) Sistema para análises estatísticas, Versão 9.0. Viçosa, Fundação Arthur Bernardes/UFV.CD-ROM.
Sadeghzadeh B & Rengel Z (2011) Zinc in Soils and Crop Nutrition. In: Hawkesford MJ & Barraclough P (Eds.) The Molecular and Physiological Basis of Nutrient Use Efficiency in Crops. Oxford, Wiley-Blackwell. p.335–375.
Sanes FSM, Castilhos RMV, Scivittaro WB, Vahl LC & Morais JR (2013) Morfologia de raízes e cinética de absorção de potássio em genótipos de arroz irrigado. Revista Brasileira de Ciências do Solo, 37:688-697.
Schäfer M, Brütting C, Baldwin IT & Kallenbach M (2016) High-Throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid- phase extraction based sample preparation with analysis by UHPLC – HESI – MS / MS. Plant Methods, 12:01-18.
Smith KA & Mullins CE (2001) Soil and environmental analysis: Physical methods. New York, Marcel Dekker. 637p.
Wilcox EG & Fageria NK (1976) Deficiências nutricionais do feijão, sua identificação e correção. Brasília, Embrapa Arroz e Feijão. 22p. (Boletim técnico, 5).
Zonta E, Brasil FC, Goi SR & Rosa MMT (2006) O sistema radicular e suas interações com o ambiente edáfico. In: Fernandes MS (Ed.) Nutrição mineral de plantas. Viçosa, Sociedade Brasileira de Ciência do Solo. p.8-52.
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