Silver nitrate, light spectrum and gas exchange on in vitro flowering of Capsicum frutescens
Palavras-chave:
light-emitting diodes, gas-permeable membranes, spectral quality, pepper production, tissue cultureResumo
The study aimed to evaluate the influence of silver nitrate on the in vitro flowering of Capsicum frutescens cv. Cayenne associated with different light spectra and gas exchange. After 36 days of germination in MS medium, two seedlings previously germinated, were subjected to experiments in glass flasks containing two types of culture medium: I) MS culture medium; or II) Culture medium with the addition of 40 µM of AgNO3. The explants were maintained in a growth room with a 16-hour photoperiod, temperature of 25 ± 2 °C, light intensity (72 μmol m-2 s-1) from different light spectra. The experiment was conducted in a completely randomized design, 4 x 2 factorial scheme, with eight replicates, and the experimental unit composed of two seedlings. After 74 days of in vitro cultivation, the following parameters were evaluated: number of bifurcations, number of oxidized buds, number of open buds, number of green buds, number of aborted buds, number of leaves, root length, shoot length, total fresh and dry mass of shoots, and total fresh and dry mass of root. Thus, the combination of light spectrum, addition of gas exchange and AgNO3 favors the growth and development of Capsicum frutescens cv. Cayenne.
Referências
Lee J. Development and Evolution of Molecular Markers and Genetic Maps in Capsicum Species. The Capsicum Genome. 2019;1(1):85–103.
FAO. Food and Agriculture Organization of the United Nations. Statistics Division [internet]. 2018. Rome: FAO; 2018. [cited: 2018 ago 22]. Available from: http://faostat3. fao.org/browse/q/qc/e.
Gris T, Pinheiro MV, Thiesen LA, Webler AR, Junges DL, Holz E, et al. Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars. Anais Acad Bras Cienc. 2021;93(3):e20190061.
Sharma A, Kumar V, Giridhar P, Ravishankar GA. Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Electron J Biotechnol. 2008;11(4):84–89.
Saldanha CW, Otoni CG, Notini MM, Kuki KN, da Cruz AC, Neto AR, et al. A CO2-enriched atmosphere improves in vitro growth of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. In Vitro Cell Dev Biol - Plant. 2013;49(5):433–444.
Batista DS, Dias LL, Rêgo MM, Saldanha CW, Otoni WC. Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants. Ciênc Rural. 2017;47(6):e20150245.
Pinheiro MV, Ríos-Ríos AM, da Cruz AC, Rocha DI, Orbes MY, Saldanha CW, et al. CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera elatior (Jack) RM Smith micropropagated plants. Braz J Bot. 2021;44(7):799–809.
Gris T, Pinheiro MV, Thiesen LA, Webler AR, Junges DL, Holz E, et al. Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars. Anais Acad Bras Cienc. 2021;93(8): e20190061.
Batista DS, Dias LL, Rêgo MM, Saldanha CW, Otoni WC. Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants. Ciênc Rural. 2017;47(9):e20150245.
Sreelekshmi R, Siril EA. Investigation on in vitro bouquets and flower longevity of micropropagated Dianthus chinensis L. Scientia Horticulturae. 2021;275(10):109708.
De Oliveira F, Gallo CM, De Oliveira JD, Santos HR, Rezende LP, Lemos EE. Nitrato de prata e tiossulfato de prata como inibidores da ação do etileno em cultivo in vitro de Annona glabra L. Diversitas Journal. 2020;5(11):2768–2778.
Pawłowska B, Żupnik M, Szewczyk-Taranek B, Cioć M. Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro. Hortic Environ Biotechnol. 2018;59(12):115–123.
Ferreira LT, Silva MM, Ulisses C, Camara TR, Willadino L. Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization. Plant Cell Tissue Organ Cult. 2017;128(13):211–221.
Manivannan A, Soundararajan P, Park YG, We H, Kim S, Jeong BR. Blue and red light-emitting diodes improve the growth and physiology of in vitro-grown carnations ‘green beauty’ and ‘purple beauty’. Hortic Environ Biotechnol. 2017;58(14):12–20.
McCree KJ. The action spectrum, absorptance and quantum yield of photosynthesis in crop plants. Agric Meteorol. 1971;9:(15)191–216.
Lazzarini LE, Pacheco FV, Silva ST, Coelho AD, Medeiros AP, Bertolucci SK, et al. Uso de diodo emissor de luz (LED) na fisiologia de plantas cultivadas–revisão. Scientia Agraria Paranaensis. 2017;16(16):137–144.
Pawłowska B, Żupnik M, Szewczyk-Taranek B, Cioć M. Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro. Hortic Environ Biotechnol. 2018;59(17):115–123.
Murashige T, Skoog F. A revised medium for a rapid growth and bioassays with tobacco tissues cultures. Physiol Plant. 1962;15(18):473–479.
Sharma A, Kumar V, Giridhar P, Ravishankar GA. Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Electron J Biotechnol. 2008;11(19):84–89.
Saldanha CW, Otoni CG, Azevedo JL, Dias LL, Rêgo MM, Otoni WC. A low-cost alternative membrane system that promotes growth in nodal cultures of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. Plant Cell Tissue Organ Cult. 2012;110(20):413–422.
Santos RP, Cruz AC, Iarema L, Kuki KN, Otoni WC. Protocolo para extração de pigmentos foliares em porta-enxertos de videira micropropagados. Rev Ceres. 2008;55(21):356–364.
Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol. 1994;144(22):307–313.
Ferreira DF. Sisvar: a computer statistical analysis system. Ciênc Agrotec. 2011;35(23):1039–1042.
Taiz L, Zeiger E, Møller IM, Murphy A. Fundamentos de Fisiologia Vegetal. 6th ed. Porto Alegre: Artmed; 2021.
Valera SV. Temperatura base, soma térmica, plastocromo e duração das fases fenológicas de cultivares de pimenta biquinho [master dissertation]. Frederico Westphalen: Universidade Federal de Santa Maria; 2017. 71 p.
da Silva JA, Kerbauy GB, Zeng S, Chen Z, Duan J. In vitro flowering of orchids. Crit Rev Biotechnol. 2014;34(26):56–76.
Gammoudi N, Pedro TS, Ferchichi A, Gisbert C. Melhoria da regeneração em pimenta: uma espécie recalcitrante. In Vitro Cell Dev Biol - Plant. 2018;54(27):145–153.
De Oliveira F, Gallo CM, De Oliveira JD, Santos HR, Rezende LP, Lemos EE. Nitrato de prata e tiossulfato de prata como inibidores da ação do etileno em cultivo in vitro de Annona glabra L. Diversitas Journal. 2020;5(28):2768–2778.
Sharma A, Kumar V, Giridhar P, Ravishankar GA. Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Electron J Biotechnol. 2008;11(29):84–89.
Haque SM, Paul S, Ghosh B. High-frequency in vitro flowering, hand-pollination and fruit setting in ten different cultivars of capsicum, spp. (C. annuum, C. chinense, and C. frutescens): an initial step towards in vitro hybrid production. Plant Cell Tissue Organ Cult. 2016;127(30):1–13.
Ribalta FM, Croser JS, Erskine W, Finnegan PM, Lulsdorf MM, Ochatt SJ. Antigibberellin-induced reduction of internode length favors in vitro flowering and seed-set in different pea genotypes. Plant Biol. 2014;58(31):39-46.
Venkatachalam P, Jinu U, Gomathi M, Mahendran D, Ahmad N, Geetha N, et al. Role of silver nitrate in plant regeneration from cotyledonary nodal segment explants of Prosopis cineraria (L.) Druce: A recalcitrant medicinal leguminous tree. Biocatal Agric Biotechnol. 2017;12(32):286–291.
Kumar GP, Sivakumar S, Siva G. Silver nitrate promotes high-frequency multiple shoot regeneration in cotton (Gossypium hirsutum L.) by inhibiting ethylene production and phenolic secretion. In Vitro Cell Dev Biol - Plant. 2016;52(33):408–418.
Aharoni N, Lieberman M. Ethylene as a regulator of senescence in tobacco leaf discs. Plant Physiol. 1979;64(34):801–804.
Davies CB, Markey CE, Busch MA, Busch KW. Determination of capsaicinoids in habanero peppers by chemometric analysis of UV spectral data. J Agric Food Chem. 2007;55(35):5925–5933.
Savvides A, Fanourakis D, van Ieperen W. Co-ordination of hydraulic and stomata conductances across light qualities in cucumber leaves. J Exp Bot. 2012;63(36):1135–1143.
Kami C, Lorrain S, Hornitschek P, Fankhauser C. Light-regulated plant growth and development. Curr Top Dev Biol. 2010;91(37):26–66.
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