Initial performance of maize in response to humic acids and plant growth-promoting bacteria
Palavras-chave:
humic substances, diazotrophic bacteria, soil organic matterResumo
Seed treatment with inoculants based on plant growth-promoting bacteria (PGPB) or the application of humic acids (HA) may increase the productivity of plants of agricultural interest. The hypothesis of this work is that it is possible to combine the effect of plant growth promoting characteristic of HA with the inoculation of PGPB selected strains in the treatment of maize seeds. Thus, providing superior responses than in single applications of both in the initial maize development. To meet this purpose, we conducted isolated application of HA or PGPB inoculation of Burkholderia gladioli and Rhizobium cellulosilyticum, and the combined application of PGPB and HA for treatment of maize seeds. At the end of the experiment (45 days after germination), the plants were evaluated biometrically, nutritionally and a bacteria count was performed in plants using the Most Probable Number technique. The results showed that it is possible to combine the effects of HA with the inoculation of selected strains of PGPB, obtaining superior responses to the isolated application of both. Thus, the use of HA-based bio-stimulants in combination with PGPB is positive and complementary compared to inputs generally used in the treatment of maize seeds.
Referências
Ahemad M & Kibret M (2014) Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University – Science, 26:1–20.
Almeida ML, Samgoi L, Nava IC, Galio J, Trentin OS & Rampazzo C (2003) Crescimento inicial do milho e sua relação com o rendimento de grãos. Ciência Rural, 33:189-194.
Alves GV, Videira SS, Urquiaga S & Reis VM (2014) Differential plant growth promotion and nitrogen fixation in two genotypes of maize by several Herbaspirillum inoculants. Plant and Soil, 386:5036-5051.
Baldotto MA, Rocha JE, Andrade FDP, Del Giúdice M. P & Baldotto LEB (2016a) Plant stimulant humic acid extracted of organic waste recycled by composting combined with liming and fertilization. Semina Ciências Agrárias, 37:3955-3964.
Baldotto MA & Baldotto LEB (2016b) Initial performance of corn in response to treatment of seeds with humic acids isolated from bokashi. Revista Ceres, 63:62-67.
Baldotto LEB, Baldotto MA, Canellas LP, Smith Reb & Olivares FL (2010) Growth promotion of pineapple ‘Vitória’ by humic acids and Burkholderia spp. during acclimatization. Revista Brasileira de Ciência do Solo, 34:1593-1600.
Baldotto MA, Muniz RC, Baldotto LEB & Dobbss LB (2011) Root growth of Arabidopsis thaliana treated with humic acids isolated from typical soils of Rio de Janeiro state, Brazil. Revista Ceres, 58:504-511.
Canellas LP & Olivares FL (2014) Physiological responses to humic substances as plant growth promoter. Chemical and Biological Technologies in Agriculture, 1:1-11.
Canellas LP, Balmori DM, Médici LO, Aguiar NO, Campostrini E, Rosa RC, Façanha AR & Olivares FLA (2012) Combination of humic substances and Herbaspirillum seropedicae inoculation enhances the growth of maize (Zea mays L.). Plant and soil, 366:119- 132
Conceição PM, Vieira HD, Canellas LP, Marques Júnior RB & Olivares FL (2008) Corn seed coating with humic acids and endophytic diazotrophic bacteria. Pesquisa Agropecuária Brasileira, 43:545-548
Cocking EC (2003) Endophytic colonization of plant roots by nitrogen-fixing bacteria. Plant and Soil, 252:169-175
Compant S, Nowak J, Coenye T, Clement C & Barka EA (2008) Diversity and occurrence of Burkholderia spp. in the natural environment. FEMS Microbiol Rev, 32:607-626.
Döbereiner J, Baldani VLD & Baldani JI (1995) Como isolar e identificar bactérias diazotróficas de plantas não-leguminosas. Brasília, Embrapa-SPI. 60p.
Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA (2001) Tratamento e manejo de dejetos de bovinos. Juíz de Fora, Embrapa. 2p. (Instrução técnica 52)
Estrada-De-Los-Santos P, Bustilios-Cristales R & Caballero-Mellado J (2001) Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Applied and Environmental Microbiology, 67:2790-2798.
García-Fraile P, Carro L, Robledo M, Ramírez-Bahena MH, Flores-Félix JD, Fernández MT, Mateos PF, Rivas R, Igua MJ, Martínez-Molina E, Peix A & Velázquez E (2012) Rhizobium promotes non-legumes growth and quality in several production steps: towards a biofertilization of edible raw vegetables healthy for humans. PLoS One, 7:38122e
Günes A, Turan M, Güllüce M & Sahin F (2014) Nutritional content analysis of plant growth-promoting rhizobacteria species. European Journal of Soil Biology, 60:88-97
Hungria M, Campo RJ, Souza EME, Pedrosa FO (2010) Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, 331:413-425.
Idris A, Labuschagne N & Korsten L (2009) Efficacy of rhizobacteria for growth promotion in sorghum under greenhouse conditions and selected modes of action studies. Journal of Agricultural Science, 147:17-30.
Long SR (2001) Genes and Signals in the Rhizobium-Legume Symbiosis. Plant Physiology, 125:69-72.
Majeed A, Abbasi MK, Hameed S, Imran A & Rahim N (2015) Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Front. Microbiol, 6:1-10.
Marques Junior RB, Canellas LP, Silva LG & Olivares FL (2008) Promoção de enraizamento de microtoletes de cana-de-açúcar pelo uso conjunto de substâncias húmicas e bactérias diazotróficas endofíticas. Revista Brasileira de Ciência do Solo, 32:1121-1128.
Masson-Boivin C, Giraud E, Perret X & Batut J (2009) Establishing nitrogen-fixing symbiosis with legumes: how many Rhizobium recipes? Trends Microbiol, 17:458-466.
Matsumura, EE, Secco VA, Moreira RS, Santos OJP, Hungria M & Oliveira ALM (2015) Composition and activity of endophytic bacterial communities in field-grown maize plants inoculated with Azospirillum brasilense. Annals of Microbiology, 1:1-14.
Melo RO, Baldotto MA & Baldotto LEB (2015) Corn initial performance in response to humic acids from bovine manure and poultry litter. Semina Ciências Agrárias, 36:1863-1874.
Montanez A, Abreu C, Gill PR, Hardarson G & Sicardi M (2009) Biological nitrogen fixation in maize (Zea mays L.) by 15N isotope dilution and identification of associated culturable diazotrophs. Biology and Fertility of Soils, 45:253-263.
Olivares FL, Aguiar NO, Rosa RCC & Canellas LP (2015) Substrate biofortification in combination with foliar sprays of plant growth promoting bacteria and humic substances boosts production of organic tomatoes. Scientia Horticulturae, 183:100-108.
Pedrosa, F (2011) Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses. Plos Genetics, 7:10002064e
Perin L, Martínez-Aguilar L, Paredes-Valdez G, Baldani JI, Estrada-De-Los-Santos P, Reis VM & Caballero-Mellado J (2006) Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize. International Journal of Systematic and Evolutionary Microbiology, 56:1931-1937.
Poonguzhali S, Madhaiyan M & Sa T (2008) Isolation and identification of phosphate solubilizing bacteria from Chinese cabbage and their effect on growth and phosphorus utilization of plants. J. Microbiol. Biotechnol, 18:773-777.
Quaggiotti S, Ruperti B, Pizzeghello D, Francioso O, Tugnoli V & Nardi S (2004) Effect of low molecular size humic substances on nitrate uptake and expression of genes involved in nitrate transport in maize (Zea mays L.). Journal of Experimental Botany, 55:803-813.
Qureshi MA, Shahzad H, Imran Z, Mushtaq M, Akhtar N, Ali MA & Mujeeb F (2013) Potential of Rhizobium species to enhance growth and fodder yield of maize in the presence and absence of l-tryptophan. The Journal of Animal & Plant Sciences, 23:1448-1454.
Ribeiro, AC, Guimarães PTG, Alvarez V (1999) Recomendação para o uso de corretivos e fertilizantes em Minas Gerais: 5ª Aproximação. Comissão de Fertilidade do Solo do Estado de Minas Gerais, Viçosa p.177.
Rodrigues Neto J (1986) Meio simples para o isolamento e cultivo de Xanthomonas campestres pv. citri Tipo B. Suma Phytopathologica, São Paulo 12:1-16 (Resumo)
Silva AC, Canellas LP, Olivares FL, Dobbss LB, Aguiar NO, Frade DOR, Rezende CE & Peres LEP (2011) Promoção do crescimento radicular de plântulas de tomateiro por substâncias húmicas isoladas de turfeiras. Revista Brasileira de Ciência do Solo, 35:1609-1617
Trevisan S, Pizzeghello D, Ruperti B, Francioso O, Sassi A, Palme K, Quaggiotti S & Nardi S (2010) Humic substances induce lateral root formation and expression. of the early auxin-responsive IAA19 gene and DR5 synthetic element in Arabidopsis. Plant Biology, 12 604- 614