Selection of progenitors for increase in oil content in soybean
Palavras-chave:
soybean, breeding, diversityResumo
The low genetic diversity brings limitation to breeding, because genetically similar genotypes share alleles in
common, causing little complementarity and low vigor due to the low levels of heterozygosity in crosses. The objective
of this work was to analyze the oil content and genetic diversity of soybean genotypes (Glycine max (L.) Merrill) based
on QTL regions of this trait for choice of progenitors for increase in oil content. Twenty-two genotypes with wide
variation in oil content, including cultivars with high oil contents, were cultivated in different Brazilian conditions and
the oil content of the grains was quantified by infrared spectrometry. Microsatellite markers selected based on QTL
regions for oil content in soybean were analyzed to estimate the genetic diversity. In these studies, a wide variation in
oil content (17.28-23.01%) and a reasonable diversity among the genotypes were observed, being PI181544 the most
divergent genotype, followed by Suprema. The genotypes PI371610/Suprema and Suprema/CD01RR8384 showed genetic
distance and higher oil contents in the grains, while the cultivars Suprema and CD01RR8384 had the highest oil contents
and proved to be little genetically related. These genotypes are promising progenitors for selection of high oil content
in soybean.
Referências
Bonato ALV, Calvo ES, Geraldi IO & Arias CAA (2006a) Genetic similarity among soybean (Glycine max (L.) Merrill) cultivars released in Brazil using AFLP markers. Genetics and Molecular Biology, 29:692-704.
Bonato ALV, Calvo ES, Arias CAA, Toledo JFF & Geraldi IO (2006b) Prediction of genetic variability through AFLP-based measure of genetic distance in soybean. Crop Breeding and Applied Biotechnology, 6:30-39.
Chen O, Zhang Z, Liu C, Xin D, Qiu H, Shan D, Shan C & Hu G (2007) QTL analysis of major agronomic traits in soybean. Agricultural Sciences in China, 6:399-405.
Chung J, Babka HL, Graef GL, Staswick PE, Lee DJ, Cregan PB, Shoemaker RC & Speech JE (2003) The seed protein, oil and yield QTL on soybean linkage group I. Crop Science, 43:1053-1067.
Csanádi G, Vollmann J, Stift G & Lelley T (2001) Seed quality QTL identified in a molecular map of early maturing soybean. Theoretical and Applied Genetics, 103:912-919.
Eskandari M, Cober ER & Rajcan I (2013) Genetic control of soybean seed oil: I. QTL and genes associated with seed oil concentration in RIL populations derived from crossing moderately high-oil parents. Theoretical and Applied Genetics, 126:483-495.
Fasoula VA, Harris DK & Boerma HR (2004) Validation and designation of quantitative trait loci for seed protein, seed oil, and seed weight from two soybean populations. Crop Science, 44:1218-1225.
Hiromoto DM & Vello NA (1986) The genetic base of Brazilian soybean Glycine max (L.) Merril) cultivars. Revista Brasileira de Genética, 9:295-306.
Hyten DL, Pantalone VR, Sams CE, Saxton AM, Landau-Ellis D, Stefaniak TR & Schmidt ME (2004) Seed quality QTL in a prominent soybean population. Theoretical and Applied Genetics, 109:552-561.
Li W, Sun D, Du Y, Chen Q, Zhang Z, Qiu L & Sun G (2007) Quantitative trait loci underlying the development of seed composition in soybean (Glycine max L. Merr.). Genome, 50:1067-1077.
Mansur LM, Orf JH, Chase K, Jarvik T, Cregan PB & Lark KG (1996) Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Science, 36:1327-1336.
Mian MR, Sung-Taeg K & Margaret GR (2009) Microsatellite diversity of soybean genotypes differing in bean pod mottle virus leaf symptom. Canadian Journal of Plant Science, 89:359-367.
Miranda ZFS, Arias CAA, Prete CEC, Kiihl RAS, Almeida LA, Toledo JFF & Destro D (2007) Genetic characterization of ninety elite soybean cultivars using coefficient of parentage. Pesquisa Agropecuária Brasileira, 42:363-369.
Mulato BM, Möller M, Zucchi MI, Quecini V & Pinheiro JB (2010) Genetic diversity in soybean germplasm identified by SSR and EST-SSR markers. Pesquisa Agropecuária Brasileira, 45:276-283.
Nichols DM, Glover KD, Carlson SR, Specht JE & Diers BW (2006) Fine mapping of a seed protein QTL on soybean linkage group I and its correlated effects on agronomic traits. Crop Science, 46:834-839.
Panthee DR, Pantalone VR, West DR, Saxton AM & Sams CE (2005) Quantitative trait loci for seed protein and oil concentration and seed size in soybean. Crop Science, 45:2015-2022.
Priolli GR, Pinheiro JB, Zucchi MI, Bajay MM & Vello NA (2010) Genetic diversity among brazilian soybean cultivars based on SSR loci and pedigree data. Brazilian Archives of Biology and Technology, 53:519-531.
Reinprecht Y, Poysa VW, Yu K, Rajcan I, Ablett GR & Pauls KP (2006) Seed and agronomic QTL in low linolenic acid, lipoxygenase free soybean germoplasm. Genome, 49:1510-1527.
Rodrigues JIS, Miranda FD, Borges LL, Silva MF, Good-God PIV, Piovesan ND, Barros EG, Cruz CD & Moreira MA (2010) QTL mapping for protein and oil content in soybeans in a Brazilian germplasm. Pesquisa Agropecuária Brasileira, 45:472-480.
Shibata M, Takayama K, Ujiie A, Yamada T, Abe J & Kitamura K (2008) Genetic relationship between lipid content and linolenic acid concentration in soybean seeds. Breeding Science, 58:361-366.
Specht JE, Chase K, Macrander M, Graef BL, Chung J, Markwell JP, Germann M, Orf JH & Lark KG (2001) Soybean response to water: a QTL analysis of drought tolerance. Crop Science, 41:493-509.
Tajuddin T, Watanabe S, Yamanaka N & Harada K (2003) Analysis of quantitative trait loci for protein and lipid contents in soybean seeds using recombinant inbred lines. Breeding Science, 55:133-140.
Vasconcelos ED, Cruz CD, Bhering LL & Resende Junior MFR (2007) Alternative method for clustering analysis. Pesquisa Agropecuária Brasileira, 42:1421-1428.
Vieira ESN, Schuster I, Silva RB, Oliveira MAR (2009) Genetic variability in soybean cultivars determined with microsatellite markers in agarose gel. Pesquisa Agropecuária Brasileira, 44:1460-1466.
Vello NA, Hiromoto DM & Azevedo Filho AJBV (1988) Coefficient of parentage and breeding of brazilian soybean germplasm. Revista Brasileira de Genética, 11:679-697.