Correlação entre a evolução de n-hexanal e aldeídos totais e a germinação e vigor de sementes de soja
Palavras-chave:
qualidade fisiológica, vigor de sementes, peroxidação lipídica, aldeídos totais, germinação de sojaResumo
Aldehyde production in soy flour was examined for its ability to indicate loss of seed viability and vigour. Five soybean seed lots, from the cultivar UFV-6, which were delay harvested at 15 days intervals after physiological maturity were tested for n-hexanal production, aldehydes assayed in the water solution after 24 hrs soaking, loss of seed germination and vigour. n-Hexanal levels in the soy flours were analysed by gas chromatography. Aldehydes in the water solution were analysed colorimetrically using MBTH as reagent. It was shown that delayed soybean harvesting after physiological maturity promotes loss of seed viability and vigour. The data presented here demonstrate a highly significative correla- tion between the amount on n-hexanal produced in the soy flour and aldehydes captured in 24 hrs of water soaking with loss of seed viability and vigour as deter- mined by standard procedures.
Referências
BENEDETTI, A.; COMPORTI, M. & ESTERBAUER, H. Identification of 4 hidroxinonenal as a citotoxic product originating from the peroxidation of liver microsomal lipids. Biochimica et Biophysica Acta., 620:281-296. 1980.
BRASIL, MINISTERIO DA AGRICULTURA. Secretaria Nacional de Defesa Agropecudria. Regras para andlise de sementes. S.1., 1980. 188 p.
CHAPMAN, G.W.Jr. & ROBERTSON, J.A. Changes in phospholipid levels during high moisture storage of soybeans. Journal of the American Oil Chemists Society. 54:195-198. 1977.
CASTRO, C.A.S.; SEDIYAMA, C.S.; MOREIRA, M.A.; SILVA, R.F. & REZEN- DE, S.T. Liberação do aldeído n-hexanal como índice para estimar o vigor de sementes de soja. Revista Ceres (aceito para publicação).
COSTA, A.V. Avaliação da Qualidade Fisiológica de Sementes de Soja (Glycine max (L.) Merrill) com Tegumento Impermeável, Produzida em Três Localidades do Brasil Central. Viçosa, Imprensa Universitária, 1984. 164 p. (Tese de Doutorado).
DELOUCHE, J.C.; STILL, T.W.; RASPET, M. & LIENHARD, M. O teste de tetrazdlio para viabilidade da semente. Brasília, AGIPLAN, 1976. 103 p.
DIANZANI, M.V. Biological activity of methylglyoxal and related aldehydes. In: HELMUTH, H.W. (ed.). Submolecular Biology and Cancer. Amsterdam, Excepta Medica, 1979. p. 245-270. (CIBA Foundation Series 67).
FEHR, W.R. & CAVINESS, C.E. Stages of Soybean Developmeni. Ames, Iowa State University, Cooperative Extention Service, 1979. 12 p.
GABRIEL, L.; BONELI, G. & DIANZANI, M.U. Inhibition of colchicine binding to rat liver tubulin by aldehydes and by linoleic acid hidroperoxide. Chemical- Biological Interactions, 19:101-109. 1977.
HARRINGTON, J.F. Seed storage and longevity. In: KOZLOWSKI, T.T. (ed.). Seed biology. New ‘York, Academic Press, 1972, v. 3. p. 145-245.
KALOYEREAS, S.A. Rancidity as a factor in the loss of viability of pine and other seeds. Journal of the American Oil Chemists Society, 35:176-179. 1958.
KITAMURA, K.; DAVIES, C.S.; KAIZUMA, N. & NIELSEN, N.C. Genetic analysis of a null-allele for lipoxygenase-3 in soybean seed. Crop Sci., 23: 924-927. 1983.
MATHEWS, S. & POWELL, A.A. Electral conductivity test. in: PERRY, D.A. (ed.). Handbook of vigour test methods. Zurick, International Testing Association, 1981. p. 3742,
PRIESTLEY, D.A. & LEOPOLD, A.C. Absence of lipid peroxidation during accelerated aging of soybean seeds. Plant Physiology, 65:245-248. 1980.
PRIESTLEY, D.A. & LEOPOLD, A.C. Lipid changes during natural aging of soybean seeds. Physiologia Plantarum. 59:467-470. 1983.
PRIESTLEY, D.A.; McBRIDE, M.B. & LEOPOLD, A.C. Tocopherol and organic free radical levels in soybean seed during natural and accelerated aging. Plant Physiology, 66:715-719. 1980.
QUEIROZ, E.E.; NEUMAIER, N.; TORRES, E.; TERAZAWA, E.; PALHANO, JB.; PEREIRA, L.A.G.; BIACHETTI, A. & YAMASHITA, J. Recomendagoes técnicas para a colheita da soja. Londrina, EMBRAPA/CNPSo, 1978. 32 p.
REZENDE, S.I. Efeito da Eliminação Genética das Lipozigenases L1 e L3 e de Características Físicas no Teor de n-Heranal em Grãos de Soja. Viçosa. Imprensa Universitária, 1986. 41 p. (Tese de Mestrado).
SEDIYAMA, T.; ARANTES, N.E.; REIS, M.S. & DHINGRA, O.D. Estudos das condições agronomicas das lavouras de soja do Triangulo Mineiro e Alto Paranafba. Minas Gerais. 1977/88. In: SEMINARIO BRASILEIRO DE PESQUISA DE SOJA. 1., Londrina. Anais... Londrina, EMBRAPA/CNPSoja, 1979. v. 1. p. 341-350.
STEWART, R.R.C. & BEWLEY, J.D. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology, 65:245-248. 1980.
TAPPEL, AL. Hematin Compounds and Lipoxidase as Biocatolysts. In: SCHULTZ, W.H. (ed). SIMPOSIUM ON FOODS. Westport, AVI Publishing Company, 1962. p. 122-138.
WILSON, D.O.Jr. & McDONALD, M.B.Jr. The lipid peroxidation model of seed aging. Seed Science and Technology, 14:269-300. 1986.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
