Evaluation of physicochemical, bioactive composition and profile of fatty acids in leaves of different olive cultivars
Palavras-chave:
nutraceutical, Olea europaea L., by productsResumo
Olive leaves are agro-industrial residues resulting from pruning and / or olive harvesting, and are used in animal feed, as an organic fertilizer and as a source of compound extraction for various applications. This study aimed to carry out the physicochemical characterization, main bioactive compounds and the fatty acid profile of olive leaves from the cultivars Frantoio, Koroneike, Manzanilha, Arbosana and Arbequina. Proximal composition, pH, titratable acidity, minerals by MIP OES, bioactive compounds were determined by spectrophotometry, oleuropein and tocopherols by high performance liquid chromatography and the fatty acid profile by gas chromatography. The olive leaves had a high content of fibers and proteins, the predominant minerals were potassium and calcium, in addition the olive leaves had a high content of bioactive compounds, mainly flavonoids and carotenoids and the cultivar Koroneike had a higher content of oleuropein compared to the others. In relation to tocopherols, á-tocopherol stood out from the other tocopherols with the maximum concentration (63436.79 mg.100g-1) measured in the cultivar Arbequina. In view of the results found, it is concluded that olive leaves are sources of macromolecules, bioactive compounds and fatty acids, which can be extracted and applied in the most diverse areas.
Referências
Abbeddou S, Rihawi S, Hess HD, Iñiguez L, Mayer AC & Kreuzer M (2011) Nutritional composition of lentil straw, vetch hay, olive leaves, and saltbush leaves and their digestibility as measured in fat-tailed sheep. Small Ruminant Research, 96:126–135.
Ahmad-Qasem MH, Barrajón-Catalán E, Micol V, Mulet A & García-Pérez JV (2013) Influence of freezing and dehydration of olive leaves (var. Serrana) on extract composition and antioxidant potential. Food Research International, 50:189–196.
AOAC - Association of Official Analytical Chemists (1995) Official Methods of analysis of AOAC International. 16th ed. Arlington, AOAC. 894p.
Bahloul N, Kechaou N & Mihoubi NB (2014) Comparative investigation of minerals, chlorophylls contents, fatty acid composition and thermal profiles of olive leaves (Olea europeae L.) as by-product. Grasas y Aceites, 65:35-43.
Ballus CA, Meinhart AD, De Souza-Campos FA, Da Silva LFDO, De Oliveira AF & Godoy HT (2014) A quantitative study on the phenolic compound, tocopherol and fatty acid contents of monovarietal virgin olive oils produced in the southeast region of Brazil. Food Research International, 62:74–83.
Bligh EG & Dyer WJ (1959) A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology, 37:911-917.
Boudhrioua N, Bahloul N, Slimen IB & Kechaou N (2009) Comparison on the total phenol contents and the color of fresh and infrared dried olive leaves. Industrial Crops and Products, 29:412–419.
Brahmi F, Mechri B, Dabbou S, Dhibi M & Hammami M (2012) The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations. Industrial Crops and Products, 38:146–152.
Brahmi F, Mechri B, Dhibi M & Hammami M (2013) Variations in phenolic compounds and antiradical scavenging activity of Olea europaea leaves and fruits extracts collected in two different seasons. Industrial Crops and Products, 49:256– 264.
Brune M, Hallberg L & Skanberg A (1991) Determination of Iron-binding Phenolic groups in Foods. Journal of Food Science, 56:128-131.
Böhmer-Maas BW, Otero DM, Zambiazi RC & Aranha BC (2020) Optimization of the extraction of phenolic compounds from olive pomace using response surface methodology. Revista Ceres, 67:181-190.
Capecka E, Mareczek A & Leja M (2005) Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry, 93:223-226.
Cavalheiro CV, Rosso VD, Paulus E, Cichoski AJ, Wagner R, Menezes CR & Barin JS (2014) Composição química de folhas de oliveira (Olea europaea L.) da região de Caçapava do Sul, RS. Ciência Rural, 44:1874-1879.
Cavalheiro CV, Picoloto RS, Cichoski AJ, Wagner R, Menezes CR, Zepka LQ, Da Croce DM & Barin JS (2015) Olive leaves offer more than phenolic compounds – Fatty acids and mineral composition of varieties from Southern Brazil. Industrial Crops and Products, 71:122–127.
Chatzistathis T, Therios I, Alifragis D & Dimassi A (2010) Effect of sampling time and soil type on Mn, Fe, Zn, Ca, Mg, K and P concentrations of olive (Olea europaea L., cv. ‘Koroneiki’) leaves. Scientia Horticulturae, 126:291-296.
Coutinho EF (2007) A cultura da Oliveira. 1st ed. Pelotas, Embrapa Clima Temperado. 143p.
Coppa CFSC, Rosin RE, De Oliveira CAF, Rodrigues CEC & Gonçalves CB (2017) Extraction of oleuropein from olive leaves using a hydroalcoholic solvent. Brazilian Journal of Food Technology, 20:e2016169.
De Oliveira MC, Ramos JD, Pio R & Das Graças-Cardoso M (2012) Características fenológicas e físicas e perfil de ácidos graxos em oliveiras no sul de Minas Gerais. Pesquisa Agropecuária Brasileira, 47:30-35.
De Vos RC, Moco S, Lommen A, Keurentjes JJ, Bino RJ & Hall RD (2007) Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry. Nature protocols, 2:778–791.
Erbay Z & Icier F (2009) Optimization of hot air drying of olive leaves using response surfasse methodology. Journal of Food Engineering, 91:533-541.
Fernández-Bolaños J, Rodríguez G, Rodríguez R, Guillén R & Jiménez A (2006) Potential use of olive by-products, Extraction of interesting organic compounds from olive oil waste. Grasas y Aceites, 57:95-106.
Fernandez-Hernandez A, Mateos R, Garcia-Mesa JA, Beltran G & Fernandez-Escobar R (2010) Determination of mineral elements in fresh olive fruits by flame atomic spectrometry. Spanish Journal of Agricultural Research, 8:1183-1190.
Funari CS & Ferro VO (2006) Análise de própolis. Ciência e Tecnologia de Alimentos, 26:171-178.
Galanakis CM, Tsatalas P & Galanakis IM (2018) Phenols from olive mill wastewater and other natural antioxidants as UV filters in sunscreens. Environmental Technology & Innovation, 9:160"168.
Gorzynik-Debicka M, Przychodzen P, Cappello F, Kuban-Jankowska A, Marino-Gammazza A & Knap N (2018). Potential health benefits of olive oil and plant polyphenols. International Journal of Molecular Sciences, 19:686.
Guo Z, Jia X, Zheng Z, Lu X, Zheng Y, Zheng B & Xiao J (2018) Chemical composition and nutritional function of olive (Olea europaea L.): a review. Phytochemistry Reviews, 17:1091–1110.
Herrero M, Temirzoda TN, Segura-Carretero A, Quirantes R, Plaza M & Ibañez E (2011) New possibilities for the valorization of olive oil by-products. Journal of Chromatography A, 1218:7511–7520.
Jabalbarezi-Hukerdi Y, Fathi MH, Rashidi L & Ganjkhanlou M (2018) The Study of Physicochemical Properties and Nutrient Composition of Mari Olive Leaf Cultivated in Iran. Nutrition and Food Sciences Research, 5:39"46.
Jane M, McKay J & Pal S (2019) Effects of daily consumption of psyllium, oat bran and polyG.ycopleX on obesity-related disease risk factors: A critical review. Nutrition, 57:84–91.
Lee OH, Lee BY, Lee J, Lee HB, Son JY, Park CS, Shetty K & Kim YC (2009) Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities. Bioresource Technology, 100:6107-6113.
Lama-Muñoz A, Contreras M del M, Espínola F, Moya M, Romero I & Castro E (2020) Content of phenolic compounds and mannitol in olive leaves extracts from six Spanish cultivars: Extraction with the Soxhlet method and pressurized liquids. Food Chemistry, 320:126626.
Lee YJ, Yang CM, Chang LW & Shen Y (2011) Effects of nitrogen status on leaf anatomy, chlorophyll content and canopy reflectance of paddy rice. Botanical Studies, 52:295–303.
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol, 148:350-381.
Lorini A, Aranha BC, Antunes BF, Otero DM, Jacques AC & Zambiazi RC (2020) Metabolic profile of olive leaves of different cultivars and collection times. Food Chemistry, 128758.
Luthria DL, Mukhopadhyay S & Kwansa AL (2006) A systematic approach for extraction of phenolic compounds using parsley (Petroselinum crispum) flakes as a model substrate. Journal of the Science of Food and Agriculture, 86:1350–1358.
Lunkes LBF & Hashizume LN (2014) Evaluation of the pH and titratable acidity of teas commercially available in Brazilian market. Revista Gaúcha de Odontologia, 62:59-64.
Martín-García AI, Moumen A, Yáñez-Ruiz DR & Molina-Alcaide E (2003) Chemical composition and nutrients availability for goats and sheep of two-stage olive cake and olive leaves. Animal Feed Science and Technology, 107:61–74.
Nogueira FAM (2012) Contribuição para caracterização de “Azeitonas de mesa mistas ao natural” produzidas de forma tradicional em Trás-os-Montes: Aspectos morfológicos, químicos e microbiológicos. Master Dissertation. Instituto Politécnico de Bragança, Bragança. 87p.
Oliveira FM, Antunes BF, Jacques AC & Zambiazi RC (2016) Teor de clorofila em folhas de oliveira cultivadas na região da campanha gaúcha. In: 15a Mostra da Produção Universitária, Rio Grande. Proceedings, Universidade Federal de Rio Grande. ISSN: 2317- 4420.
Oreste EQ, Jesus A, Oliveira RM, Silva MM, Vieira MA & Ribeiro AS (2013) New design of cold finger for sample preparation in open system: determination of Hg in biological samples by CV-AAS. Microchemical Journal, 109:5-9.
Orak HH, Isbilir SS & Yagar H (2012) Determination of Antioxidant Properties of Lyophilized Olive Leaf Water Extracts Obtained from 21 Different Cultivars. Food Science Biotechnology, 21:1065-1074.
Otero DM, Oliveira FM, Lorini A, Antunes BF, Oliveira RM & Zambiazi RC (2020) Oleuropein: Methods for extraction, purifying and applying. Revista Ceres, 67:240-254.
Pacetta CF (2013) Estudo de diferentes metodologias para obtenção de extratos de folhas de oliveira (Olea europaea) contendo Oleuropeína. Master Dissertation. Universidade de São Paulo, Pirassununga. 82p.
Price ML, Scoyoc SV & Butler LG (1978) A Critical Evaluation of the Vanillin Reaction as an Assay for Tannin in Sorghum Grain. Journal Agriculture Food Chemistry, 26:1214-1218.
Quirantes-Piné R, Lozano-Sánchez J, Herrero M, Ibáñez E, Segura-Carretero A & Fernández-Gutiérrez A (2012) HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts. Phytochemical Analysis, 24:213–223.
Rahmanian N, Jafari SM & Wani TA (2015) Bioactive profile, dehydration, extraction and application of the bioactive components of olive leaves. Trends in Food Science and Technology, 42:150–172.
Rodriguez-Amaya DB (2001) A guide to carotenoid analysis in food. Washington, International Life Sciences Institute. 64p.
Rosa AD, Junges A, Fernandes IA, Cansian RL, Corazza ML, Franceschi E, Backes GT & Valduga E (2019) High pressure extraction of olive leaves (Olea europaea): bioactive compounds, bioactivity and kinetic modelling. Journal of Food Science and Technology, 56:3864–3876.
Sahan Y, Basoglu F & Gucer S (2007) ICP-MS analysis of a series of metals (namely: Mg, Cr, Co, Ni, Fe, Cu, Zn, Sn, Cd and Pb) in black and green olive samples from Bursa, Turkey. Food Chemistry, 105:395–399.
Kubola J & Siriamornpun S (2008) Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chemistry, 110:881–890.
Talhaoui N, Taamalli A, Gómez-Caravaca AM, Fernández-Gutiérrez A & Segura-Carretero A. (2015) Phenolic compounds in olive leaves: Analytical determination, biotic and abiotic influence, and health benefits. Food Research International, 77:92–108.
Tarchoune I, Sgherri C, Eddouzi J, Zinnai A, Quartacci MF & Zarrouk M (2019) Olive leaf addition increases olive oil nutraceutical properties. Molecules, 24:545.
Tan H, Tuck K, Stupans I & Hayball P (2003) Simultaneous determination of oleuropein and hydroxytyrosol in rat plasma using liquid chromatography with fluorescence detection. Journal of Chromatography B, 785:187-191.
Visioli F & Galli C (2002) Biological properties of olive oil phytochemicals. Critical Reviews in Food Science and Nutrition, 42:209-221.
Zambiazi RC (1997) The role of endogenous lipid components on vegetable oil stability. Doctoral Thesis. University of Manitoba, Winnipeg. 304p.
Downloads
Publicado
Como Citar
Edição
Seção
Licença

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.