Endophytic bacteria in seed germination and rooting of Pinus spp.
Keywords:
adventitious rooting, Azospirillum brasilense, germination, Pinus sp., plant growth-promoting bacteriaAbstract
The inoculation of seeds with associative and growth-promoting bacteria is a prosperous mechanism to achieve high germinability rates and production of well-developed plants, in addition to the aspects related to the rhizogenic process in the clonal propagation of superior genotypes. Consequently, the objective of this work was to isolate endophytic bacteria from Pinus caribaea var. hondurensis plant tissues and evaluate its potential as a promoter in seed germination and rooting of P. taeda mini-cuttings. Hence, endophytic bacteria were isolated from Pinus caribaea var. hondurensis micro-plants grown in vitro and phenotypically characterized. From this collection of formed endophytic isolates, in addition to two Azospirillum brasilense commercial strains, seed germination and rooting tests of Pinus taeda L. mini-cuttings were established. Bacterial inoculation promoted the germination rate, germination speed and vigor of the seedlings. A. brasilense and CNPF 316 promoted an increase in the percentage of rooted mini-cuttings, number and average length of roots. The isolates present characteristics of plant growth-promoting bacteria, as they enhance the development of plant physiological and morphological stages.
References
Amjad H, Shafqat F, Nayyer I & Rubina A (2004) Influence of exogenous application of hydrogen peroxide on root and seedling growth on wheat (Triticum aestivum L.). International Journal of Agricultural and Biological Engineering, 6:366-369.
Brader G, Compant S, Mitter B, Trognitz F & Sessitsch A (2014) Metabolic potential of endophytic bacteria. Current Opinion in Biotechnology, 27:30-37.
Cassán F, Perrig D, Sgroy V, Masciarelli O, Penna C & Luna V (2009) Azospirillum brasilense az39 and Bradyrhizobium japonicum e109, inoculated singlyor in combination, promote seed germination and early seedling growth in corn (Zeamays L.) and soybean (Glycine max L.). European Journal of Soil Biology, 45:28-35.
Çavusoglu K & Kabar K (2010) Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. EurAsian Journal of Biosciences, 4:70-79.
Dartora J, Guimarães VF, Marini D, Pinto Júnior AS, Cruz LM & Mensch R (2013) Influência do tratamento de sementes no desenvolvimento inicial de plântulas de milho e trigo inoculados com Azospirillum brasiliense. Scientia Agraria Paranaensis, 12:175-181.
Döbereiner J, Baldani VLD & Baldani JI (1995) Como isolar e identificar bactérias diazotróficas de plantas não-leguminosas. Brasília, Embrapa - SPI. 60p.
Duarte MM, Moraes RF, Martin DM & Zuffellato-Ribas KC (2020) Potencial de utilização de Azospirillum brasilense e ácido indolbutírico no enraizamento de estacas de jasmim-amarelo. Advances in Forestry Science, 7:889-895.
IBÁ - Indústria Brasileira de Árvores (2020) São Paulo, Relatório IBÁ. 121p.
Ishizawa H, Kuroda M, Morikawa M & Ike M (2017) Evaluation of environmental bacterial communities as a factor affecting the growth of duckweed Lemna minor. Biotechnology for Biofuels, 10:62.
Jesus JS, Dorléans VR, Ribeiro DP, Sodré GA & Barbosa RM (2020) Mini-cuttings of forest and fruit species. Científica, 48:67-75.
Labouriau LG (1983) A germinação das sementes - Série de Biologia. 24ª ed. Washington, Secretaria Geral da Organização dos Estados Americanos, Programa Regional de Desenvolvimento Científico e Tecnológico. 174p.
Labouriau LG & Valadares MEB (1976) On the germination of seeds of Calotropis procera (Ait.) Ait. f. Anais da Academia Brasileira de Ciências, 48:263-284.
Maguire JD (1962) Speed of germination – aid in selection and evaluation for seedling emergence and vigor. Crop Science, 1:176-177.
Liotti RG, Figueiredo MIS, Silva GF, Mendonça EAF & Soares MA (2018) Diversity of cultivable bacterial endophytes in Paullinia cupana and their potential for plant growth promotion and phytopathogen control. Microbiological Research, 207:08-18.
Mariosa TN, Melloni EGP, Melloni R, Ferreira GMR, de Souza SMP & da Silva LFO (2017) Rizobactérias e desenvolvimento de mudas a partir de estacas semilenhosas de oliveira (Olea europeae L.). Revista de Ciências Agrárias, 60:302-306.
Marques E, Aquiles KR, Blum LEB & Uesugi CH (2014) Bactérias extremófilas facultativas melhorando a germinabilidade de sementes de Eucalyptus urophylla s.t. Blake. Revista Árvore, 38:489-494.
Panigrahi S, Mohanty S & Rath CC (2020) Characterization of endophytic bacteria Enterobacter cloacae MG00145 isolated from Ocimum sanctum with indole acetic acid (IAA) production and plant growth promoting capabilities against selected crops. South African Journal of Botany, 134:17-26.
Quisen RC & Degenhardt-Goldbach J (2020) Metodologia de descontaminação e germinação de sementes de Pinus taeda L. Colombo, Embrapa. 6p. (Comunicado Técnico, 449).
Rosa DD, Villa F, Silva DF & Corbari F (2018) Rooting of semihardwood cuttings of olive: indolbutyric acid, calcium and Azospirillum brasilense. Comunicata Scientiae, 9:34-40.
Santos RF, Cruz SP, Botelho GR & Flores AV (2018) Inoculation of Pinus taeda seedlings with plant growth-promoting rhizobacteria. Floresta e Ambiente, 25:e20160056.
Santoyo G, Moreno-Hagelsieb G, Orozco-Mosqueda MC & Glick BR (2016) Plant growth-promoting bacterial endophytes. Microbiological Research, 183:92-99.
Sharma S, Yadav S & Sibi G (2020) Seed germination and maturation under the influence of hydrogen peroxide – a review. Journal of Critical Reviews, 7:06-10.
Silva FAS & Azevedo CAV (2016) The Assistat software version 7.7 and its use in the analysis of experimental data. African Journal of Agricultural Research, 11:3733-3740.
Stadler JA, Lopes ES, Rodrigues CK, Oliveira FM & Diniz CCC (2022) Harvester productivity and costs in clear cutting Pinus taeda stands under different management regimes. Floresta, 52:189-196.
Vandana UK, Rajkumari J, Singha LP, Satish L, Alavilli H, Sudheer PDVN, Chauhan S, Ratnala R, Satturu V, Mazumder PB & Pandey P (2021) The endophytic microbiome as a hotspot of synergistic interactions with prospects of plant growth promotion. Biology, 10:101.
Wojtyla L, Lechowska K, Kubala S & Garnczarska M (2016) Different modes of hydrogen peroxide action during seed germination. Frontiers in Plant Science, 7:66.
Zul D, Elviana M, Ismi KRN, Tassyah KR, Siregar BA, Gafur A & Tjahjono B (2022) Potential of PGPR isolated from rhizosphere of pulpwood trees in stimulating the growth of Eucalyptus pellita F. Muell. International Journal of Agriculture Technology, 18:401-420.
