Reproductive studies in commercial cocoa tree

Authors

  • Pabliane Ramos Lawinscky Universidade Estadual de Santa Cruz
  • Margarete Magalhães Souza Universidade Estadual de Santa Cruz
  • Cláusio Antônio Ferreira de Melo Universidade Estadual de Santa Cruz
  • Dário Ahnert Universidade Estadual de Santa Cruz
  • Luiz Roberto Martins Pinto Universidade Estadual de Santa Cruz
  • Ronan Xavier Corrêa Universidade Estadual de Santa Cruz
  • Marc Ernso Georges Universidade Estadual de Santa Cruz

Keywords:

Theobroma cacao, stigma receptivity, pollen viability, clone TSH1188, clone CCN51

Abstract

Theobroma cacao is a species of economic importance mainly for the food industry. The objective of the present study was to analyze pollen viability, stigma receptivity and controlled pollinations as a function of flower opening time (nine times, from 8:00 am to 4:00 pm) in selected plants of clones TSH1188 and CCN51. There was a higher average percentage of stigma receptivity except at the last collection time (4:00 pm). The clones showed a higher average percentage of pollen viability from 8:00 to 11:00 am. Fertilization percentage varied from 12.5 to 68.7%  in clones throughout the flower opening time; a gradual trend in the fall in fertilization percentage was observed until 12:00 pm. Significant differences between clones for pollen viability and stigma receptivity were observed, indicating the influence of controlled pollination time on these characteristics.

References

1. Almeida AA, Valle RR. Ecophysiology of the cacao tree. Braz J Plant Physiol. 2007;19(4):425-48.

2. Alvim PT. Flowering of cocoa. Cocoa Growers' Bull. 1984;25:23-31.

3. Bartley BGD. The genetic diversity of cacao and its utilization. Cambridge: CABI Publishing; 2005.

4. Chapman RK, Soria SJ. Comparative Forcypomya (Diptera, Ceratopogonidae) pollination of cacao in Central America and Southern Mexico. Rev Theobroma. 1983;13(2):129-39.

5. Salazar-Díaz R, Torres-Coto V. Study of the dynamics of cocoa (Theobroma cacao) pollinators in three production systems. Tecnol Marcha. 2017;30(1):90-100.

6. Maia-Silva C, Hrncir M, Giannini TC, Toledo-Hernández M, Imperatriz-Fonseca VL. Small Amazonian stingless bees: an opportunity for targeted cocoa pollination. Front Bee Sci. 2024;2:1357811. https://doi.org/10.3389/frbee.2024.1357811

7. Lanaud C, Fouet O, Legavre T, Lopes U, Sounigo O, Eyango M, et al. Deciphering the Theobroma cacao self-incompatibility system:from genomics to diagnostic markers for self-incompatibility. J Exp Bot. 2017;68(17):4775-90. https://doi.org/10.1093/jxb/erx293

8. Müller MW, Valle RR. Ecofisiologia do cultivo do Cacaueiro. In: Valle RR, editor. Ciência, tecnologia e manejo do Cacaueiro. Itabuna: Gráfica e Editora Vital Ltda.; 2007. p. 17-41.

9. Cadima A. Efeito da água subterrânea sobre a produção de cacau. Cacau Atual. 1971;8:8-10.

10. Meinhardt LW, Rincones J, Bailey BA, Aime MC, Griffith GW, Zhang D, et al. Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao: what's new from this old foe? Mol Plant Pathol. 2008;9(5):577-88.

11. Lopes UV, Monteiro WR, Marques JRB, Pires JL, Gramacho KP. Participatory cacao clones evaluation for resistance to witches’ broom in agrosystems from Bahia, Brazil. In: Proceedings of the 14th International Cocoa Research Conference; 2003 Oct 9-14; Accra, Ghana. Accra: Cocoa Research Institute of Ghana; 2005. p. 993-9.

12. Campo EC, Andía FC. Cultivo y beneficio del cacao CCN 51. Quito: El Conejo; 1973.

13. Morán CQ, Schettini GN, Ochoa DF, González AB, Cantos IS, Jazayeri SM. Selection of self-compatible progenies of high productivity cocoa in the Ecuadorian coast. Cienc Tecnol. 2021;14(1):11-21.

14. Omolaja SS, Aikpokpodion PO, Adedeji A, Vwioko D. Rainfall and temperature effects on flowering and pollen productions in cocoa. Afr Crop Sci J. 2009;17(1):41-8.

15. Arenas-De-Souza MD, Rossi APB, Varella TL, Silveira GF, Souza SAM. Stigmatic receptivity and pollen viability of Theobroma subincanum Mart.: fruit species from the Amazon region. Rev Bras Frutic. 2016;38(4):e-757. https://doi.org/10.1590/0100-29452016757

16. Kearns CA, Inouye DW. Techniques for pollination biologists. Niwot: University Press of Colorado; 1993.

17. Pearse AGE. Histochemistry, theoretical and applied. 3rd ed. London: Churchill Livingstone; 1972.

18. Alexander MP. Differential staining of aborted and non-aborted pollen. Stain Technol. 1969;44(3):117-22.

19. Dafni A. Pollination ecology: a practical approach. New York: Oxford University Press; 1992.

20. Boza EJ, Motamayor J, Amores F, Cadeño-Amador S, Tondo CL, Livingstone DS, et al. Genetic characterization of the cacao cultivar 'CCN 51' and its impact and significance on global cacao improvement and production. J Am Soc Hortic Sci. 2014;139(2):219-29.

21. Wickramasuriya AM, Dunwell JM. Cacao biotechnology: current status and future prospects. Plant Biotechnol J. 2017;16(1):4-17.

22. Souza MM, Pereira TNS, Martins ER. Microsporogenesis and microgametogenesis with relation to flower bud and anther size, and pollen viability in yellow passion fruit (Passiflora edulis Sims f. flavicarpa Degener). Cienc Agrotec. 2002;26(6):1209-17.

23. Belo GO, Souza MM, Souza VO, Melo CAF. Reproductive and Cytogenetic characterization in Passiflora sublanceolata. Biologia. 2015;70(6):733-43.

24. Borges HBN. Biologia reprodutiva de Centrosema pubescens Benth. (Fabaceae). Bol Mus Para Emílio Goeldi Cienc Nat. 2006;1(1):31-8.

25. Stiehl-Alves EM, Martins MP. Biologia reprodutiva de Acacia Mearnsii De Wild.: receptividade de estigmas. Rev Árvore. 2008;32(4):609-16.

26. Santos AO, Webber AC, Costa FRC. Biologia reprodutiva de Psychotria spectabilis Steyrm. e Palicourea cf. virens (Poepp & Endl.) Standl. (Rubiaceae) em uma floresta tropical úmida na região de Manaus, AM, Brasil. Acta Bot Bras. 2008;22(1):275-85.

27. Krieck C, Finatto T, Müller TS, Guerra MP. Reproductive biology of Alpinia zerumbet (Pers.) B. L. Burtt & R. M. Sm. (Zingiberaceae) in Florianópolis, Santa Catarina State, Brazil. Rev Bras Plantas Med. 2008;10(2):103-10.

28. Cope FW. The mechanism of pollen incompatibility in Theobroma cacao L. Heredity. 1962;17(2):157-82.

29. Souza MM, Pereira TNS, Viana AP, Pereira MG, Bernacci LC, Sudré CP, et al. Meiotic irregularities and pollen viability in Passiflora edmundoi Saco (Passifloraceae). Caryologia. 2003;56(2):161-9.

30. Falque M, Kodia AA, Sounigo O, Eskes AB, Charrier A. Gamma-irradiation of cacao (Theobroma cacao L.) pollen: effect on pollen grain viability, germination and mitosis and on fruit set. Euphytica. 1992;64(3):167-72.

31. Motamayor JC, Risterucci AM, Lopez PA, Ortiz CF, Moreno A, Lanaud C. Cacao domestication I: the origin of the cacao cultivated by the Mayas. Heredity. 2002;89(5):380-6.

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Published

2026-08-21

How to Cite

Lawinscky, P. R., Souza, M. M., Ferreira de Melo, C. A., Ahnert, D., Pinto, L. R. M., Corrêa, R. X., & Georges, M. E. (2026). Reproductive studies in commercial cocoa tree. Revista Ceres, 73, e773015. Retrieved from https://ojs.ceres.ufv.br/ceres/article/view/8242

Issue

Vol. 73 (2026)

Section

PHYSIOLOGY, ECOPHYSIOLOGY, AND DEVELOPMENT OF CULTIVATED PLANTS

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