Action of ethylene on postharvest of summer squash ‘Menina Brasileira’
Resumen
The purpose of this study was to evaluate the sensitivity and the physiological responses of summer squash
‘Menina Brasileira’ to ethylene. Immature fruits were harvested and placed in 20 L sealed buckets, in which ethylene was
applied at concentrations of 0.1, 1.0, 10, 100, and 1000 μL L-1 for 24 h. Fruits were placed in buckets with no ethylene as
a control treatment. Thereafter, the fruits were taken out of the buckets and maintained on bench, wherein on days 0, 2,
4, 6, and 8, they were evaluated regarding the accumulated fresh weight loss, soluble sugars, reducing and non-reducing
sugars, starch, total chlorophyll, content of malondialdehyde, and electrolyte leakage. Fruits of summer squash ‘Menina
Brasileira’ showed sensitivity to exogenous ethylene with no weight loss stimulation. Additionally, the fruits exhibited
small changes in nutritional quality attributes and changes in the external fruit appearance, including decreased chlorophyll
content as well as damage to cell membrane characterized by increase in malondialdehyde content and electrolyte
leakage. These changes were stimulated by increasing exogenous ethylene concentration.
Citas
Barry CS & Giovannoni JJ (2007) Ethylene and fruit ripening. Journal of Plant Growth Regulation, 26:143-159. https://dx.doi.org/10.1007/s00344-007-9002-y
Cakmak I & Horst WJ (1991) Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 83:463-468. https://dx.doi.org/10.1111/j.1399-3054.1991.tb00121.x
Cao S, Yang Z & Zheng Y (2012) Effect of 1-methylcyclopropene on senescence and quality maintenance of green bell pepper fruit during storage at 20 ºC. Postharvest Biology and Technology, 70:1-6. http://dx.doi.org/10.1016/j.postharvbio.2012.03.005
Carmo GA (2009) Crescimento, nutrição e produção de cucurbitáceas cultivadas sob diferentes níveis de salinidade da água de irrigação e doses de adubação nitrogenada. Tese de Doutorado–Universidade Federal Rural do Semiárido, Mossoró. 183 p.
Chitarra MIF & Chitarra AB (2005) Pós-colheita de frutos e hortaliças: fisiologia e manuseio. 2ª ed. Lavras: UFLA. 785p.
Dubois M, Gilles KA, Hamilton JK, Rebers PA & Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28:350-356. http://dx.doi.org/10.1021/ac60111a017
Finger, FL & Vieira G (2007) Controle da perda pós-colheita de água em produtos hortícolas. Caderno Didáticos 19. Viçosa- MG, UFV. 29 p.
Heath RL & Packer L (1968) Photoperoxidation in isolated chloroplast: I- Kinetics and stoichometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125:189-198. http://dx.doi.org/10.1016/0003-9861(68)90654-1
Inskeep WP & Bloom PR (1985) Extinction coefficients of chlorophyll a and bin N, N – Dimethylformamide and 80% acetone. Plant Physiology, 7:483-485.
Kader AA (2002) Postharvest technology of horticultural crops. 3ª ed. University of California, Agriculture and Natural Resources, Publication 3311. 535p.
Lima ALS, daMatta FM, Pinheiro HA, Totola, MR & Loureiro ME (2002) Photochemical responses and oxidative stress in two clones of Coffea canephora under water deficit conditions. Environmental and Experimental Botany, 47:239-247.
Maia VM, Salomao LCC, Siqueira DL, Aspiazu I & Maia LCB (2014) Physical and metabolic changes induced by mechanical damage in 'Dwarf-Prata' banana fruits kept under cold storage [online]. Australian Journal of Crop Science, 8(7): 1029-1037.
McAtee P, Karim S, Schaffer R & David K (2013) A dynamic interplay between phytohormones is required for fruit development, maturation, and ripening. Frontiers in Plant Science, 4:79. http://dx.doi.org/10.3389/fpls.2013.00079
McCready RM, Guggolz J, Silveira,V & Owens HS (1950) Determination of starch and amylose in vegetables. Analytic Chemistry, 22:1156-1158. http://dx.doi.org/10.1021/ac60045a016
Nelson N (1944) A photometrric adaptation of the Somogyi method for the determination of glucose.The Journal of Biological Chemistry, 153:375-380.
Paul V, Pandey R & Srivastava GC (2012) The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene – an overview. Journal of Food Science and Technology, 49:1–21. http://dx.doi.org/10.1007/s13197-011-0293-4
Universidade Federal de Viçosa (2007) SAEG: Sistema para Análises Estatísticas e Genéticas. Versão 9.1. Viçosa, Fundação Arthur Bernardes. CD-ROM.
Stevenson DG, Eller FJ, Wang L, Jane J, Wang T & Inglett GE (2007) Oil and tocopherol content and composition of pumpkin seed oil in 12 Cultivars. Journal of Agricultural and Food Chemistry, 55(10): 4005–4013.
Taiz L & Zeiger E (2013) Fisiologia vegetal. 5. ed. Porto Alegre, Artmed. 918p.
Yamane M, Abe D, Yasui S, Yokotani N, Kimata W, Ushijima K, Nakano R, Kubo Y & Inaba A (2007) Differential expression of ethylene biosynthetic genes in climacteric and non-climacteric Chinese pear fruit. Postharvest Biology and Technology, 44:220-227. http://dx.doi.org/10.1016/j.postharvbio.2006.12.010
Yoo SD, Cho Y & Sheen J (2009) Emerging connections in the ethylene signaling network. Trends in Plant Science, 14:270–279. http://dx.doi.org/10.1016/j.tplants.2009.02.007
