Bacillus subtilis no tratamento de mudas de bananeira infectadas por fitonematoides
Palabras clave:
biological control, Musa spp., rhizobacteria, treatment of plantletsResumen
The use of healthy propagative plant material is an important management method for nematodes in banana crops (Musa spp.), once it prevents the introduction of nematodes in non-infested areas. The aim of this work was to evaluate the effect of the bio-nematicide Nemathel® (Bacillus subtilis) on Terra banana plantlets cv. Comprida infected with a mixed population of Radopholus similis, Meloidogyne spp., Pratylenchus spp., and Helicotylenchus spp. Different doses of Nemathel® (50, 100, 150, 200, and 250 mL/10 L water) were tested in addition to the positive (nematicide) and negative (water) treatments. The plantlets were immersed in each treatment for 30 min and subsequently, planted in pots containing sterilized soil in greenhouse. The evaluation was performed four months later. Dosages of 200 and 250 mL of Nemathel®/10 L in water showed the greatest reduction of nematode populations in roots and rhizomes.
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Araújo FF & Marchesi GVP (2009) Uso de Bacillus subtilis no controle da meloidoginose e na promoção de crescimento do tomateiro. Ciência Rural, 39:1558-1561.
Blomme G, Beule H, Swennen RL, Tenkouano A & Waele D (2004) Effect of nematodes on root and shoot growth of in vitro propagated and sword sucker-derived plants of six Musa spp. genotypes. Nematology, 6:593-604.
Cardozo RB & Araújo FF (2011) Multiplicação de Bacillus subtilis em vinhaça e viabilidade no controle da meloidoginose, em cana-de-açúcar. Revista Brasileira de Engenharia Agrícola e Ambiental, 15:1283-1288.
Coolen WA & D´Herde CJ (1972) A method for the quantitative extraction of nematodes from plant tissue. Ghent, State Agricultural Research Center. 77p.
Cordeiro ZJM, Matos AP & Kimati H (2016) Doenças da bananeira. In: Amorim L, Rezende JAM, Bergamin Filho A & Camargo LEA (Eds.) Manual de Fitopatologia: doenças das plantas cultivadas. 5ªed. Ouro Fino, Agronômica Ceres. p.109-123.
Fernandes RH, Vieira BS, Fuga CAG & Lopes EA (2014) Pochonia clamydosporia e Bacillus subtilis no controle de Meloidogyne incognita e M. javanica em mudas de tomateiro. Bioscience Journal, 30:194-200.
Ferraz S, Freitas LG, Lopes EA & Dias-Arieira CR (2010) Manejo sustentável de fitonematoides. Viçosa, Editora UFV. 306p.
Gowen SR, Quénéhervé P & Fogain R (2005) Nematodes of banana and plantains. In: Luc M, Sikora R & Bridge J (Eds.) Plant parasitic nematodes in subtropical and tropical agriculture. 2ª ed. London, CAB International. p.611-643.
Guimarães CP (2011) Controle biológico de fitonematoides na cultura da banana no norte de Minas Gerais. Dissertação de Mestrado. Universidade Estadual de Montes Claros, Montes Claros. 90p.
Jenkins WR (1964) A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporter, 48:692.
Lopes OS (2011) Aplicação de rizobactérias em explantes e plântulas de bananeira ‘prata-anã’ no controle de Meloidogyne javanica e no desenvolvimento de mudas. Dissertação de Mestrado. Universidade Estadual de Montes Claros, Montes Claros. 123p.
Ludwig J, Moura AB & Gomes CB (2013) Potencial da microbiolização de sementes de arroz com rizobactérias para o biocontrole do nematoide das galhas. Tropical Plant Pathology, 38:264-268.
Mai WF & Mullin PG (1996) Plant-parasitic nematodes: a pictorial key to genera. 5th ed. New York, Cornell University. 277p.
Mekete T, Dababat A, Sekora N, Akyazi F & Abebe E (2012) Identification key for agriculturally important plant-parasitic nematodes: a manual for nematology. Mexico, CIMMYT. 22p.
Mutua GK, Karanja NK, Ayuke F, Ndukhu H & Kimenju JW (2011) The potential of Bacillus subtilis and Rhizobium leguminosarum in controlling plant-parasitic nematodes in farmers’ fields. African Crop Science Conference Proceedings, 10:209-215.
Oostenbrink M (1966) Major characteristics of the relation between nematodes and plants. Wageningen, Mededelingen Landbouwhogeschool. 46p.
Ribeiro RCF, Campos VP, Xavier AA, Rocha LS, Ribeiro HB, Aguiar FM, Souza RM, Mizobutsi EH & Dias-Arieira CR (2012) Rizobactérias no controle de Meloidogyne javanica e mal do Panamá em bananeira. Nematropica, 42:218-226.
