Produção e acúmulo de substâncias com características de fitoalexina em folhas de fumo inoculadas do patógeno incompatível Pseudomonas syringue, pv. psi, com relação ao tempo
Palavras-chave:
resposta hipersensível, substâncias antimicrobianas, fitoalexinas foliares, patógeno incompatível, bioensaio em placasResumo
Tobacco leaves were innoculated with the incompatible pathogen Pseudomo- nas syringae pv. pisi by using the leaf infiltration technique. After 0, 6, 12, 24, 48, and 72 hours infil‘rated leaf tissue was harvested and extracted by the facilitated diffusion technique in 40% ethanol. The diffusates were concentrated in a flask evaporator and extracted with ethyl acetate. Quantification of antimicrobial activity in the extracts was then performed by using bioassays against Thiela- viopsis paradoza (bicautography and paper disc method) and Corynebacterium michiganense pv. michiganense (paper disc method). The concentration of antimi- crobial compounds was undetectable at zero time but showed a tendency to increase after some hours, remaining stable after 12-24 hours.
Referências
BAILEY, J.A. & MANSFIELD, J.W. Phytoalezins. London, Blackie & Sons, 1982. 334 p.
BAILEY, J.A.; VINCENT, G.G. & BURDEN, R.S. Diterpenes from Nicotiana glutinosa and their effect on fungal growth. Journal of General Microbiology, 85:87-94. 1974.
CHEEMA, A.S. & HAARD, N.F. Induction of rishitin and lubimin in potato tuber discs by non-specific elecitors and the influence of storage conditions. Physiological Plant Pathology, 13:233-240. 1978.
CRUICKSHANK, LA.M. Phytoalexins. Annual Review of Phytopathology, 1: 351-374. 1963.
GNANAMANICKAM, S.S. & MITH, D.A. Selective toxicity of isoflavonoid phytoalexins to gram-positive bacteria. Phytopathology, 70:894-896. 1980.
KEEN, N.T. Phytoalexins: efficient extraction from leaves by a facilited diffusion technique. Phytopathology, 68:1237-1239. 1978,
KEEN, N.T. The isolation of phytoalexins from germinating seeds of Cicer arietinum, Vigna sinensis, Arachis hypogea, and other plants. Phytopathology, 75:91-92. 1975.
KEEN, N.T. TLC bioassay for antifungal substances using Cladosporium cucumerinum. Phytopathology, 61:1084. 1971.
KIRALY, Z.; KLEMENT, Z; SOLYMOSY, F. & VOROS, J. Methods in Plant Pathology. Budapest, Kiadó, 1970. 508 p.
KLEMENT, Z. & GOODMANN, R.N. The hypersensitive reactions to infection by bacterial plant pathogens. Annual Review of Phytopathology, 5:17-44. 1967.
KUC, J.A. Phytoalexins. Annual Review of Phytopathology, 10:207-232. 1972.
LYON, F.M. & WOOD, R.K.S. Production of phaseollin, cumestrol and related compounds in bean leaves inoculz‘ed with Pseudomonas spp. Physiological Plant Pathology, 6:117-124. 1975.
MANSFIELD, J.W. Mechanism of Resistance to Botrytis. In: COLEY-SMITH, J.R.; JARVIS W.R. & VERHOEFTF, K. (ed.). The Biology of Botrytis. New York, Academic Press, 1980. p. 181-218.
O’ BRIEN, F. & WOOD, R.K.S. Role of ammonia in infection of Phaseolus vulgaris by Pseudomonas spp. Physiological Plant Pathology, 3:315-325, 1973.
REILLY, J.J. & KLARMAN, W.L. The soybean hydroxiphaseollin induced by fungicides. Phytopathology, 62:1113-1115. 1972.
ROMEIRO, R.S. Identificação de Bactérias Fitopatogénicas. Viçosa, Imp. Univ., UFV, 1976. 91 p.
ROMEIRO, R.S. Reação de hipersensibilidade induzida por bactérias fitopatogeénicas. Seiva, 33:1340. 1973.
SILVA, T.D. Produção de substancias com características de fitoalezinas em resposta a inoculação de Pseudomonas syringae pv. pisi em plantas de fumo. Viçosa, UFV, 1982. 53 p. (Tese de Mestrado).
SILVA, T.D. & ROMEIRO, R.S. Possível envolvimento de fitoalexinas na resposta hipersensivel de plantas de fumo (Nicotiana tabacum) a inoculação com Pseudomonas Ssyringae pv. pisi. Fitopatologia Brasileira 7:558, 1982. (Abstract).
TUITE, J. Plant Pathological Methods: Fungi and Bacteria. Minneapolis, Burgess, 1969. 239 p.
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