Moderate doses of selenium alleviate the effects of salt stress on the growth of eucalyptus seedlings
Palavras-chave:
sustainable forestry, salinity, gas exchange and growthResumo
Although selenium (Se) is not essential for plant metabolism, low Se concentrations can enhance tolerance to abiotic stress. This study evaluated the effects of exogenous Se (1, 5, and 25 μM) on two eucalyptus genotypes (VCC 865 and AEC 0144) under saline stress (100 mM NaCl). Growth, biomass, gas exchange, ion accumulation (K+, Na+, Ca2+), and pigment content were analyzed. Salinity significantly impaired physiological performance, reducing height, stem diameter, net photosynthesis (PN), transpiration (Tr), and stomatal conductance (gs), while increasing Na+ in roots and leaves. Se at 1 and 5 μM mitigated these effects, particularly by reducing Na+ accumulation. VCC 865 showed increased K+ in roots and decreased Na+ in shoots with 1 μM Se, while AEC 0144 displayed improved ionic balance in roots and leaves. The chlorophyll pigment content remained unchanged between the treatments. However, 25 μM Se intensified salinity damage, indicating the importance of dose optimization. Photosynthetic parameters were sensitive indicators of Se-mediated responses. Therefore, the targeted use of selenium represents a promising strategy to improve seedling establishment in environments affected by salinity, contributing to the development of more resilient forest systems and the expansion of areas suitable for production.
Referências
1. Flora e Funga do Brasil. Eucalyptus in Flora e Funga do Brasil. Rio de Janeiro: Jardim Botânico do Rio de Janeiro; 2023.
2. IBÁ – Indústria Brasileira de Árvores. Relatório IBÁ 2022. São Paulo: IBÁ; 2022.
3. Lorenzi HJ, Bacher LB, Torres MAV. Árvores e arvoretas exóticas no Brasil: madeireiras, ornamentais e aromáticas. Nova Odessa: Instituto Plantarum de Estudos da Flora; 2018.
4. Reis LAC, de Oliveira JA, dos Santos Farnese F, Rosado AM, Reis LAC. Chlorophyll fluorescence and water content parameters are good biomarkers for selecting drought tolerant eucalyptus clones. For Ecol Manag. 2021;481:118682.
5. Cunha TQG, Santos AC, Novaes E, Hansted ALS, Yamaji FM, Sette Jr CR. Eucalyptus expansion in Brazil: Energy yield in new forest frontiers. Biomass Bioenergy. 2021;144:105900.
6. Shrivastava P, Kumar R. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J Biol Sci. 2015;22(2):123-31.
7. FAO – Food and Agriculture Organization of the United Nations. World map of salt-affected soils launched at virtual conference. Roma: FAO; 2021.
8. Zhao C, Zhang H, Song C, Zhu JK, Shabala S. Mechanisms of plant responses and adaptation to soil salinity. Innovation (N Y). 2020;1(1):100017.
9. Srivastava P, Wu QS, Giri B. Salinity: an overview. In: Giri B, Wu QS, editors. Microorganisms in Saline Environments: Strategies and Functions. Cham: Springer; 2019. p. 3-18.
10. Dias NS, Blanco FF, Souza ER, Ferreira JFS, Neto ONS, Queiroz Í. Efeitos dos sais na planta e tolerância das culturas à salinidade. In: Gheyi HR, Dias NS, Lacerda CF, editors. Manejo da salinidade na agricultura: estudos básicos e aplicados. Fortaleza: INCTSal; 2016. p. 151-62.
11. Tripathi DK, Singh S, Singh S, Chauhan DK, Dubey NK, Prasad R. Silicon as a beneficial element to combat the adverse effect of drought in agricultural crops: capabilities and future possibilities.Water Stress Crop Plants Sustain Approach. 2016;2:682-94.
12. Dong X, Sun L, Guo J, Liu L, Han G, Wang B. Exogenous boron alleviates growth inhibition by NaCl stress by reducing Cl⁻ uptake in sugar beet (Beta vulgaris). Plant Soil. 2021;464(1-2):423-39.
13. Sohag AAM, Tahjib-Ul-Arif M, Brestic M, Afrin S, Sakil MA, Hossain MT, Hossain MA. Exogenous salicylic acid and hydrogen peroxide attenuate drought stress in rice. Plant Soil Environ. 2020;66(1):7-13.
14. Azizi I, Esmaielpour B, Fatemi H. Effect of foliar application of selenium on morphological and physiological indices of savory (Satureja hortensis) under cadmium stress. Food Sci Nutr. 2020;8(12):6539-49.
15. Santos Junior RN, Faria-Silva L, Falqueto AR, Werner ET. Selenium enhances photosynthetic efficiency in eucalyptus under saline stress. S Afr J Bot. 2025;183:13-22.
16. Gupta M, Gupta S. An overview of selenium uptake, metabolism, and toxicity in plants. Front Plant Sci. 2017;7:2074.
17. Jiang C, Zu C, Lu D, Zheng Q, Shen J, Wang H, Li D. Effect of exogenous selenium supply on photosynthesis, Na⁺ accumulation and antioxidative capacity of maize (Zea mays L.) under salinity stress. Sci Rep. 2017;7:42039.
18. Terry N, Zayed AM, De Souza MP, Tarun AS. Selenium in higher plants. Annu Rev Plant Biol. 2000;51:401-32.
19. Clark RB. Characterization of phosphatase of intact maize roots. J Agric Food Chem. 1975;23(3):458-60.
20. Hawrylak-Nowak B. Beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress. Biol Trace Elem Res. 2009;132:259-69.
21. Lacerda JJ. Estresse Salino e Seus Efeitos no Crescimento Inicial de Clones de Eucalyptus spp. [dissertação]. Vitória da Conquista: Universidade Estadual do Sudoeste da Bahia; 2016.
22. Malavolta E, Vitti GC, Oliveira SAD. Avaliação do estado nutricional das plantas: princípios e aplicações. 2a ed. Piracicaba: Potafos; 1997.
23. Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol. 1994;144(3):307-13.
24. Islam F, Farooq MA, Gill RA, Wang J, Yang C, Ali B, Wang G, Zhou W. 2,4-D attenuates salinity-induced toxicity by mediating anatomical changes, antioxidant capacity and cation transporters in the roots of rice cultivars. Sci Rep. 2017;7:10443.
25. Schmoeckel SM, Jarvis DE. Salt stress. In: Kole C, editor. Genomic Designing of Climate-Smart Cereal Crops. Cham: Springer; 2017. p. 1-25.
26. Willadino L, Camara T. Tolerância das plantas à salinidade: aspectos fisiológicos e bioquímicos. Encicl Biosf. 2010;6(11):1-23.
27. Ji H, Pardo JM, Batelli G, van Oosten MJ, Bressan RA, Li X. The salt overly sensitive (SOS) pathway: established and emerging roles. Mol Plant. 2013;6(2):275-86.
28. Kwon OK, Mekapogu M, Kim KS. Effect of salinity stress on photosynthesis and related physiological responses in carnation (Dianthus caryophyllus). Hortic Environ Biotechnol. 2019;60:831-39.
29. Botelho VV, Lacerda JDJ, Cairo PAR, Barbosa RP. Efeito da salinidade sobre características morfológicas de clones de Eucalyptus spp. durante o crescimento inicial. Semana Agron UESB SEAGRUS. 2017;1(1):1-10.
30. Castro Filho MN, Tagliaferre C, Oliveira FS, Silva BL, Alves RO, Paula AD, Barros FM. Biomass of two Eucalyptus clones (E. grandis × E. urophylla) irrigated with saline water. Rev Arvore. 2022;46:e4607.
31. Hasanuzzaman M, Bhuyan MB, Raza A, Hawrylak-Nowak B, Matraszek-Gawron R, Al Mahmud J, Nahar K, Fujita M. Selenium in plants: Boon or bane? Environ Exp Bot. 2020;178:104170.
32. Brown TA, Shrift A. Selenium: toxicity and tolerance in higher plants. Biol Rev Camb Philos Soc. 1982;57(1):59-84.
33. Feng R, Wei C, Tu S. The roles of selenium in protecting plants against abiotic stresses. Environ Exp Bot. 2013;87:58-68.
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