Eficiência relativa da técnica de flutuação centrífuga, usando-se diferentes líquidos de separação, velocidades de centrifugação e caulim, para extrair fitonematóides de três tipos de solo
Palavras-chave:
flutuação centrífuga, líquido de separação, textura do solo, recuperação de nematoides, solução de açúcarResumo
Several modifications were made in the centrifugal flotation technigue in an attempt to increase efficiency in extracting plant parasitic nematodes from soil samples.
Referências
ALLEN, O.N. Experiments in soil bacteriology. Minneapolis, Burgess, 1959, 117p.
ALVARADO 8., M. & LOPEZ C., R. Extracciéon de nematodos fitoparésitos asociados al arroz, cv. C.R. 1113, mediante modificaciones de las técnicas de centrifugacion-flotacion y embudo de Baermann modificado. In: RESEARCH AND PLANNING CONFERENCE ON ROOT-KNOT NEMATODES, Meloi- dogyne spp., 3%, Panama, 1982. Proceedings, Panamá City, IDIAP, 1982. p.35-47.
ALVARADO S., M. & LOPEZ C., R. Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del embudo de Baermann modificado y centrifugacion-flotacion. Turrialba 32(1): 83-87. 1982,
AYALA, A.; ROMAN, J. & TARJAN, A.C. Comparison of four methods for isolating nematodes from soil samples. The Journal of Agriculture of the University of Puerto Rico 47(4):219-225. 1963.
BARKER, K.R. Root-Knot Workshop — Extraction. S.., s. ed., 1983. 8 p.
BARKER, K.R.; NUSBAUM, CJ. & NELSON, L.A. Seasonal population dynamics of selected plant-parasitic nematodes as measured by three extraction procedures. Journal of Nematology 1(3):232-239. 1969.
BRAVO, M.A.C. Eficácia do método de flutuação centrífuga na extração de nematodos de pequenas dimensões. Agronomia Lusitana 34(4):283-291. 1972.
BRAVO, M.A.C. Comparison of the Christie and Perry and the centrifugalflotation methods for extracting Xiphinema mediterraneum from soil. Agronomia Lusitana 38(3):203-212. 1977.
CAVENESS, F.E. & JENSEN, H.J. Modification of the centrifugal-flotation technique for the isolation and concentration of nematodes and eggs from soil and plant tissue. Proceedings of the Helminthological Society of Washington 22(2):87-89. 1955.
COOLEN, W.A. Methods for the extraction of Meloidogyne spp. and other nematodes from roots and soil. In: LAMBERTI, F. & TAYLOR, C.E., ed. Rootknot nematodes (Meloidogyne species); Systematics, Biology and Control. London, Academic Press, Inc., 1979. p. 317-329.
COOLEN, W.A. & D'HERDE, C.J. Extraction de Longidorus et Xiphinema spp. du sol par centrifugation en utilisant du silice coloidal. Nematologia Mediterranea 5(2):195-206. 1977.
DECRAEMER, W.; COOLEN, W.A. & HENDRICK, G.J. Evaluation of extraction methods for a survey of Trichodoridae in fields of the seed potatoes. Nematologica 25:494-495. 1975.
DICKERSON, O.J. An evaluation of the direct centrifugal-flotation method of recovering nematodes from soil. Plant Disease Reporter 61(12):1054-1057. 1971.
GOORIS, J. & D'HERDE, C.J. A method for the quantitative extraction of eggs second stage juveniles of Meloidogyne spp. from soil. Belgium, Min. Agric., State Nematol. Res. Station, 1972. 36 p.
HARRISON, JM. & GREEN, C.D. Comparison of centrifugal and other methods for standardization of extraction of nematodes from soil. Annual of Applied Biology 82(2):299-308. 1976.
JENKINS, W.R. A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporter 48(9):692. 1964.
MENDES, M.L.; FERRAZ, S. & REGAZZI, A.J. Eficiéncia de seis métodos de extração na recuperagao de nematéides de diferentes géneros em trés tipos de solo. Revista Ceres 35(197):1-16. 1988.
RODRIGUEZ-KABANA, R. & KING, P.S. Efficiency of extraction of nematodes by flotation-sieving using molasses and sugar and by elutriation. Journal of Nematology 7(1):54-59. 1975.
RODRIGUEZ-KABANA, R. & KING, P.S. The use of sugarcane molasses as an economical substitute for sugar in the extraction of nematodes from soil by the flotation-sieving technique. Plant Disease Reporter 56(12):1093-1096. 1972.
VIGLIERCHIO, D.R. & SCHMITT, R.V. On the methodology of nematode extraction from field samples: comparison of methods for soil extraction. Journal of Nematology 15(3):450-454. 1983.
VIGLIERCHIO, D.R. & YAMASHITA, T.T. On the methodology of nematode extraction from field samples: density flotation technique. Journal of Nematology 15(3):444-449. 1983.
VOLCY, C. Velocidad y tiempo de centrifugacion para extraer nematodos fitoparasitos del suelo. Rev. Faculdad Nacional de Agronomia Medellin 33(1): 15-20. 1980.
VOLCY, C. Evaluacion de métodos de extraccion de nematodos en suelos de diferentes texturas. Fitopatologia Colombiana 7(1):15-18. 1978.
WEBER, D.E. & WILLIAMS, A.S. Evaluatién of the centrifugal-flotation technique for the quantitative recovery of three types of ectoparasitic nematodes. Nematologica 14(1):18. 1968.
WEHUNT, E.J. Sodium-containing detergents enhance the extraction of nematodes. Journal of Nematology 5(1):79-80. 1973.
WILLARD, J.R. & PETROVICH, M.S. Efficiency of the centrifugal-flotation method for extraction of nematodes from clay soils. Plant Disease Reporter 59(4):314-318. 1975.
WILLARD, J.R. & PETROVICH, M.S. A direct centrifugal-flotation method for extraction of nematode from clay soils. Plant Disease Reporter 56(9):808-810. 1972.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
