Productivity and nutritive value of Tifton 85 bermudagrass inoculated with Azospirillum brasilense in association with nitrogen fertilization

Authors

  • Aline Rodrigues Silva UFSM
  • Clair Jorge Olivo UFSM
  • Diego Martins Granada UFSM
  • Lucas Giovane Casagrande UFSM
  • Mauricio Pase Quatrin UFSM
  • Caroline Paim Sauter UFSM

Keywords:

crude protein, Cynodon spp., diazotrophic bacteria, forage yield, total digestible nutrients

Abstract

Tifton 85 bermudagrass has been widely used in tropical and subtropical regions. Owing to its high forage yield, the fertilizer requirement of Tifton 85 is also high. Inoculation with Azospirillum brasilense is an alternative to reduce the use of nitrogen fertilizers and decrease their environmental impact. However, the effects of inoculation in tropical grasses remain poorly understood. This study aimed to evaluate the productivity, pasture characteristics, and nutritive value of Tifton 85 bermudagrass inoculated with A. brasilense in association with nitrogen fertilization. The study evaluated four nitrogen levels (0, 100, 200, and 300 kg ha−1), inoculated and uninoculated, under cutting conditions. The experimental design was completely randomized, in a factorial arrangement, with two qualitative (inoculated with A. brasilense or uninoculated) and four quantitative levels (nitrogen doses) with three replications (24 plots). Botanical composition and morphological characteristics, leaf blade/stem+sheath ratio, nutritive value, and forage yield were evaluated. The inoculation increased the leaf blade/stem+sheath ratio and forage yield of Tifton 85, but did not affect crude protein concentration, neutral detergent fiber content, and total digestible nutrient content of the forage constituted by Tifton 85 leaf blades. The protein concentration and forage production corresponded linearly to the nitrogen dose increase.

References

Aguirre PF, Giacomini SJ, Olivo CJ, Bratz VF, Quatrin MP & Schaefer GL (2020) Biological nitrogen fixation and urea-N recovery in ‘Coastcross-1’ pasture treated with Azospirillum brasilense. Pesquisa Agropecuária Brasileira, 55:01-10.

Aguirre PF, Olivo CJ, Rodrigues PF, Falk DR, Adams CB & Schiafino HP (2018a) Forage yield of Coastcross-1 pastures inoculated with Azospirillum brasilense. Acta Scientiarum: Animal Sciences, 40:01-08.

Aguirre PF, Olivo CJ, Sauthier JC, Sauter MP, Aires JF, Seibt DC & Simonetti GD (2018b) Valor nutritivo da Coastcross-1 inoculada com Azospirillum brasilense. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70:1997-2006.

Alderman PD, Bootle KJ & Sollenberger LE (2011) Regrowth dynamics of ‘Tifton 85’ bermudagrass as affected by nitrogen fertilization. Crop Science, 51:1716-1726.

Almeida JC de C, Morais LF de, Moreira TGB, Abreu JBR de & Morenz MJF (2019) Nutritive value of Tifton 85 hay ammoniated with urea. Acta Scientiarum: Animal Sciences, 41:01-7.

Anderson W & Stewart M (2017) Tifton 85 bermudagrass response to fertilization on two coastal plain soils. Better Crops With Plant Food, 4:24-26.

Andrade RA, Porto MO, Cavali J, Ferreira E, Bergamin AC, Souza FR de & Aguiar IS de (2019) Azospirillum brasilense e fosfato natural reativo no estabelecimento de forrageira tropical. Revista de Ciências Agrárias, 42:146-154.

Anjos ANS dos, Olivo CJ, Sauter CP, Silva AR, Santos FT dos & Seibt DC (2016) Forage yield in pastures with bermudagrass mixed with different legumes. Acta Scientiarum: Animal Sciences, 38:261-266.

AOAC - Association of Official Analytical Chemists (1995) Official methods of analysis. 16th ed. Washington, AOAC. 1015p.

Baseggio M, Newman Y, Sollenberger LE, Fraisse C & Obreza T (2015) Planting rate and depth effects on Tifton 85 bermudagrass establishment using rhizomes. Crop Science, 55:1338-1345.

Bashan Y & Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth – A critical assessment. Advances in Agronomy, 108:77-136.

Borges BMMN, Silveira ML, Cardoso SS, Moline EFV, Coutinho Neto AM, Lucas FT, Muraoka T & Coutinho ELM (2017) Growth, herbage accumulation, and nutritive value of ‘Tifton 85’ bermudagrass as affected by nitrogen fertilization strategies. Crop Science, 57:01-10.

Bourscheidt MLB, Pedreira BC, Pereira DH, Zanette MC & Devens J (2019) Estratégias de fornecimento de nitrogênio em pastagens: fertilizante mineral, inoculante bacteriano e consórcio com amendoim forrageiro. Scientific Electronic Archives, 12:137-147.

Burton GW (2001) Tifton 85 Bermudagrass – early History of its creation, selection, and evaluation. Crop Science, 41:05-06.

Cappelle ER, Valadares Filho S de C, Silva JFC da & Cecon PR (2001) Estimates of the energy value from chemical characteristics of the feedstuffs. Revista Brasileira de Zootecnia, 30:1837-1856.

Cunha FN, Silva NF da, Bastos FJ de C, Carvalho JJ de, Moura LM de F, Teixeira MB, Rocha AC da & Souchie EL (2014) Efeito da Azospirillum brasilense na produtividade de milho no Sudoeste goiano. Revista Brasileira de Milho e Sorgo, 13:261-272.

Díaz-Zorita M & Fernandez-Canigia MV (2009) Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity. European Journal of Soil Biology, 45:03-11.

Dobbelaere S, Vanderleyden J & Okon Y (2003) Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences, 22:107-149.

Galindo FS, Teixeira Filho MCM, Tarsitano MAA, Buzetti S, Santini JMK, Ludkiewicz MGZ, Alves CJ & Arf O (2017) Economic analysis of corn inoculated with Azospirillum brasilense associated with nitrogen sources and doses. Semina: Ciências Agrárias, 38:1749-1764.

Guimarães SL, Bonfim-Silva EM Polizel AC & Campos DT da S (2011) Produção de Capim-Marandu inoculado com Azospirillum spp. Enciclopédia Biosfera, 7:816-825.

Hungria M, Campo RJ, Souza EM & Pedrosa FO (2010) Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil, 331:413-425.

Hungria M, Nogueira MA & Araujo RS (2016) Inoculation of Brachiaria spp. with the plant growth-promoting bacterium Azospirillum brasilense: An environment-friendly component in the reclamation of degraded pastures in the tropics. Agriculture, Ecosystems and Environment, 221:125-131.

INMET – Instituto Nacional de Meteorologia (2018) Banco de dados meteorológicos para ensino e pesquisa. Dados mensais Estação Meteorológica de Santa Maria – Cód. A803.

Lana MC, Dartora J, Marini D & Hann JE (2012) Inoculation with Azospirillum, associated with nitrogen fertilization in maize. Revista Ceres, 59:399-405.

Leite R da C, Santos JGD dos, Silva EL, Alves CRCR, Hungria M, Leite R da C & Santos AC dos (2019) Productivity increase, reduction of nitrogen fertilizer use and drought-stress mitigation by inoculation of Marandu grass (Urochloa brizantha) with Azospirillum brasilense. Crop Pasture & Science, 70:61-67.

Licea-Herrera JI, Quiroz-Velasquez JC & Hernandez-Mendoza JL (2020) Impact of Azospirillum brasilense, a rhizobacterium stimulating the production of indole-3-acetic acid as the mechanism of improving plants’ grow in agricultural crops. Revista Boliviana de Química, 37:34-39.

Michelangeli JAC, Newman YC, Sollenberger LE, Staples C, Ortega LE & Christiman MC (2010) Managing harvest of ‘Tifton 85’ bermudagrass for production and nutritive value. Forage and Grazinglands, 8:01-08.

Morais TP de, Brito CH de, Ferreira A de S & Luz JMQ (2015) Aspectos morfofisiológicos de plantas de milho e bioquímico do solo em resposta à adubação nitrogenada e à inoculação com Azospirillum brasilense. Revista Ceres, 62:507-509.

Moreira FM de S, Silva K da, Nóbrega RSA & Carvalho F de (2010) Bactérias diazotróficas associativas: diversidade, ecologia e potencial de aplicações. Comunicata Scientiae, 1:74-79.

Mumbach GL, Kotowski IE, Schneider FJA, Mallmann MS, Bonfada EB, Portela VO, Bonfada EB & Kaiser DR (2017) Resposta da inoculação com Azospirillum brasilense nas culturas de trigo e de milho safrinha. Scientia Agraria, 18:97-103.

Nascimento MTCC do, Azevedo CAV de, Santos JS dos, Lima VLA de & Barbosa RBG (2017) Crescimento e produção do capim Tifton 85 irrigado com água residuária e adubação orgânica. Espacios, 38:13-24.

Oliveira APP, Rossiello ROP, Galzerano L, Costa Júnior JBG, Silva RP & Morenz MJF (2010) Respostas do capim-Tifton 85 à aplicação de nitrogênio: cobertura do solo, índice de área foliar e interceptação da radiação solar. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 62:429-438.

Olivo CJ, Agnolin CA, Bratz VF, Diehl MS, Simonetti GD, Correa M da R, Rodrigues PF, Fantineli DG, Nunes JS & Bem CM de (2014) Produtividade de pastos consorciados com leguminosas forrageiras. Revista de Agricultura, 89:78-90.

Olivo CJ, Quatrin MP, Sauter CP, Silva AR, Sauthier JC & Sauter MP (2019) Productivity and crude protein concentration of Tifton 85 pasture based mixed with pinto peanut. Ciência e Agrotecnologia, 43:01-08.

Pereira OG, Rovetta R, Ribeiro KG, Santos MER, Fonseca DM & Cecon PR (2012) Crescimento do capim-tifton 85 sob doses de nitrogênio e alturas de corte. Revista Brasileira de Zootecnia, 41:30-35.

Quatrin MP, Olivo CJ, Simonetti GD, Bratz VF, Godoy GL de & Casagrande LG (2019) Response of dual-purpose wheat to nitrogen fertilization and seed inoculation with Azospirillum brasilense. Ciência e Agrotecnologia, 43:01-10.

Sabundjian MT, Arf O, Kaneko FH & Ferreira JP (2013) Adubação nitrogenada em feijoeiro em sucessão a cultivo solteiro e consorciado de milho e Urochloa ruziziensis. Pesquisa Agropecuária Tropical, 43:0292-299.

Santos MS, Nogueira MA & Hungria M (2021) Outstanding impact of Azospirillum brasilense strains Ab-V5 and Ab-V6 on the Brazilian agriculture: Lessons that farmers are receptive to adopt new microbial inoculant. Revista Brasileira de Ciência do Solo, 45:01-31.

Silva ACC da, Lima LA, Almeida WF de, Thebaldi MS & Silva AC da (2017) Tifton 85 production under deficit irrigation. Revista de la Facultad de Ciencia Agrarias, 49:117-126.

Sohm G, Thompson C, Assefa Y, Schlegel A & Holman J (2014) Yield and quality of irrigated bermudagrass as a function of nitrogen rate. Agronomy Journal, 106:1489-1496.

Soil Survey Staff (2014) Keys to Soil Taxonomy. 12th ed. Washington, USDA – Natural Resources Conservation Service. 372p.

SAS Institute Inc. (2001) Statistical Analysis System user’s guide. Version 8.2. Cary, Statistical Analysis System Institute. 1686p.

Tedesco MJ, Gianello C, Anghinoni I, Bissani CA, Camargo FAO & Wiethölter S (2004) Manual de adubação e de calagem para os estados do Rio Grande do Sul e de Santa Catarina. 10a ed. Porto Alegre, Sociedade Brasileira de Ciência do Solo, Núcleo Regional Sul. 400p.

Van Soest PJ, Robertson JB & Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and no starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74:3583-3597.

Vogel GF, Martinkoski L, Martins PJ & Bichel A (2013) Desempenho agronômico de Azospirillum brasilense na cultura do arroz: uma revisão. Revista em Agronegócios e Meio Ambiente, 6:567-578.

Zanine A de M, Nascimento Júnior D do, Santos MER, Pena K da S, Silva SC da & Sbrissia AF (2011) Características estruturais e acúmulo de forragem em Capim-Tanzânia sob pastejo rotativo. Revista Brasileira de Zootecnia, 40:2364-2373.

Downloads

Published

2025-06-04

How to Cite

Rodrigues Silva, A., Jorge Olivo, C., Martins Granada, D., Casagrande, L. G., Pase Quatrin, M., & Paim Sauter, C. (2025). Productivity and nutritive value of Tifton 85 bermudagrass inoculated with Azospirillum brasilense in association with nitrogen fertilization. Revista Ceres, 70(4), 42–50. Retrieved from https://ojs.ceres.ufv.br/ceres/article/view/8125

Issue

Section

CROP PRODUCTION

Most read articles by the same author(s)