Vitamina C em frutos de interesse tecnológico (IV. Comunicação sobre vitamina C)
Palavras-chave:
ácido ascórbico, vitamina c, teor total, método rotineiro, substâncias interferentesResumo
By means of adaptation for routine work we analised total vitamin C contents (reduced ascorbic acid plus dehydroascorbic acid)
by condensation of its oxydized form with 2,4 dinitrophenylhydrazin (DNP). The color developed by the condensation product was strongly influenced by the sulfuric acid concentration whenused to solubilize the DNP-derivate.
Referências
BRUNE, W., BATISTA, C.M., SILVA, D.O., FORTES, J.M. & PINHEIRO, R.V. Sobre o teor de vitamina C em Mirtáceas. Rev. Ceres, Viçosa, 13(74):123-133. 1966.
BRUNE, W., SILVA, D.O. & MATTOS, J. Sobre o teor de vitamina C em Mirtáceas II. Rev. Ceres, Viçosa, 13(75):182-193. 1966.
HAWK, P.B., OSER, B.L. & SUMMERSON, W.H. Practical Physiological Chemistry. 132 ed., New York, McGraw Hill Book. 1954. 1440 p.
KOLLER, S. Graphische Tafeln zur Beurteilung Statistischer Zahlen, 28 ed., Varlag von T. Steinkopff. Dresden. 1943. 75 p.
ROE, J. H. Comparative analyses for Ascorbic Acid bythe2,4 Dinitrophenylhydrazin method the coupling reaction at different temperatures: a procedure for determining specificity, J. Biol. Chem., Baltimore, 236(5):1611-1613. 1961.
ROE, J.H. & KUETHER, C. A. A color reaction for Dehydroascorbic acid useful in determination of vitamin C. Science. New York, 95(2453):77. 1942.
