Análise genética de endospermas translúcidos originados de plantas homozigóticas para o gene opaco-2 em milho (Zea mays L.)
Keywords:
endosperma translúcido, gene opaco-2, mutação induzida, teor de lisina, radiação gamaAbstract
From experiments in which opaque-2 maize seeds were treated with gamma rays and ethil methanesulfonate, and their respective untreated controls, seeds with hard, vitreous endosperms were obtained. Some of these were completely vitreous, with no evidence of opaque endosperm tissue. Others had very small and few (one to three) areas of opaque tissue. Plants derived from completely vitreous endosperm seeds were self pollinated and crossed to an opaque-2 inbred. The segregation of vitreous to opaque seeds indicated that the normal allele at the opaque-2 locus was responsible for the vitreousity of the endosperm. Lysine content of the vitreous endosperm was comparable to that of normal endosperms. Plants derived from vitreous seeds with few and tiny spots of opaque tissue pro- duced, upon selfing or crossing to the opaque-2 inbred, only opaque-2 seeds.
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